Dear List members, We are planning to work with Sperm Chromatin Structure Assay using Acridine Orange. I have some queries for those who have worked in this area and have published papers, 1) When you talk about red fluorescence does that mean FL-3 values? and the same for the Green fluorescence meaning the FL-1 values? 2) So, does the Total Fluorescence mean FL1 values + FL3 values? 3) I got hold of the raw data from a FITC-PSA and EthD experiment. When I asked the machine (computer with FACS) to calculate FL-1 + FL-3 (Probably the Total fluorescence) values for me and then FL-3/FL1+FL3 (Probably the alpha-t), it gave me totally different values then what I calculated using the EXCEL. So I got confused as to what exactly do we mean by total fluorescence, is it FL1+FL3 or does it take into consideration some other parameters too. However, the computer calculated and self (excel) calculated Total fluorescence and alpha-t values were highly correlated. I hope I am clear in my questions. Rahul Rahul Rathi BVSc, MS, PhD Dept of Clinical Sciences, Faculty of Veterinary Medicine, Tel : 785-532-4413/5700 Kansas State University, Fax: 785-532-4989 Manhattan, 66505, KS rrathi@vet.ksu.edu USA "Research is to see what everybody else has seen, and to think what nobody else has thought"- Albert Szent-Gyorgyi --------------------------------- Do you Yahoo!? Faith Hill - Exclusive Performances, Videos, & more faith.yahoo.com
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:27 EST