[Coordinator's note: The attachment with this message is an FCS data file. ] [ Steve ] I have a sample of periphereal blood, 40 years, woman, WBC 82.700. In flow cytometric analysis, there are four populations: R1: CD19(-), CD20(-), CD5(-), CD2(-), CD3(-), CD7(-),CD61(-), CD41(-), CD10(-), TdT(-), CD14(-), CD16(-), CD56(-), CD33 (+++), CD13(+++), MPO(++) (mean fluorescence: 25.36), CD34 (++), HLA-DR (-). The second region, R2, the same size, but more granular (vacuoles?): CD19(-), CD20(-), CD5(-), CD2(-), CD3(-), CD7(-),CD61(-), CD41(-), CD10(+/- 17.85 %), TdT(-), CD14(-), CD16(+/- 25.01%), CD56(-), CD33 (+++), CD13(+++), MPO(+++) (mean fluorescence: 50.43), CD34 (-), HLA-DR (-). The third region, R3 looks like normal granulocites, and the fourth region looks like normal lymphocytes. I think that R1 may be a M3 leukemia HLA-DR(-), but R2, may be also a M3 HLA-DR(-)?, Is only one kind of leukemia?, or is biphenotype? In attachment, I send some files that are ineteresting I hope some help Thanks -- JUAN LUIS CASTILLO N. CENTRO DIAGNOSTICO ONCOINMUNOLOGICO LTDA. CITOMETRIA DE FLUJO HOSPITAL DEL TRABAJADOR CARDENIO AVELLO 36 CONCEPCION CHILE PHONE: 56-41-402138 56-41-329454 FAX: 56-41-402117 E-MAIL: axelyoyi@entelchile.net <mailto:axelyoyi@entelchile.net>
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