We are doing some immunophenotyping with cyno monkey blood. We are evaluating the stability of the blood at room temperature and using Streck stabilizer (stored at 4C). All of our cell populations remain stable except we see diminished B cells as early as 24 hours when samples are collected in EDTA or heparin tubes. Has anyone else had similar issues? We are still evaluating our stabilized samples but with our results to date we have an occasional animal where the B cells are much lower after 24 or more hours in stabilizer than when we processed the blood immediately. I know for human work folks use samples collected in heparin and I have not seen reports of decreased B cells when samples are tested 24 hours after collection. If anyone else has had similar issues I would be interested in hearing from you. Deborah LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
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