On 7/2/02 10:55 PM, "Houston, Jim" <Jim.Houston@stjude.org> wrote: > > To All: > > Anyone have some information about detecting Langerhan's by Flow? Any > info/direction would be helpful. > > Jim Houston For human LC or epidermal dendritic cells, check out the combo HLA-DR+ CD1a+ E-cadherin+ CCR6+ while FactorXIIIa neg. CD14 neg. in contrast to dermal DC (and also CD3 neg. CD19 neg. CD16/56 neg.). S100 is a marker often used in immunohistochemistry of LC histiocytosis, but I don't know if it's been tried on Flow. Mice LC are also Lin- and have relatively abundant MHCII, plus also surface CD11c, F4/80 and E-cadherin but no CD1a on murine DC. Regards, Karim
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