Marty, See attached spect shifts of a variety of ptn FL. Stephen P. Perfetto, MS.,MT. (ASCP) Manager, Core Flow Cytometry Facility Vaccine Research Center, NIH40 Convent Dr., Room 5507Bethesda, MD 20892-3015 <<CFP-Spectra.PPT>> > ---------- > From: Marty Bigos > Sent: Tuesday, August 13, 2002 10:49 AM > To: Cytometry Mailing List > Subject: Re: gfp spectral shifts? > > > >Perhaps another (highly intersting) shift-inducing parameter exists > >: protein maturation. It is known that newly produced dsRed, a > >distant GFP homolog protein has a "greener" emission spectrum than > >the mature dsRed-protein. This might be the case for GFP as well ... > > > >Jan > > Interesting! Do you have a reference on this? We have used dsRed here > only a little so haven't seen this behavior. > > Thanks. > > Marty > > > > >Marty Bigos wrote: > > > >> > >>Hi - > >> > >>Does anyone know of shifts in the emission spectra of the various gfp > >>constructs due to intracellular pH, linkage to other proteins, etc.? > >>Thanks. > >> > >> > >>Marty > >>-- > >>Marty Bigos > >>Director, Flow Core > >>Gladstone Institute of Virology and Immunology > >>Building 3 SFGH Rm 509 > >>415-695-3832 > >> > > > >-- > >Jan Bayer, Ph.D. > >Fondation Jean Dausset - CEPH Email : bayer@cephb.fr > >27, Rue Juliette Dodu Tel : +33 1 53 72 51 14 > >75010 Paris, France Fax : +33 1 53 72 51 28 > >
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