Hi Serotec make anti-Rat ED1 antibodies (unconjugated, FITC or biotinylated). This is a good marker for rat tissue macrophages but it is also expressed on monocytes. Best wishes Simon Watson Phagocyte Lab University of Edinburgh UK ----- Original Message ----- From: "Polchert, David" <Polchert@uic.edu> To: cyto-inbox Sent: Tuesday, August 27, 2002 11:28 PM Subject: Macrophage presence in rat cell line? > > Hello All, > > We have been testing a rat cell line for phagocytosis. Although the > particular cell line is not known to be related to macrophages, we suspect > their presence due to possible phagocytosis taking place. Can anyone > suggest the best way to detect rat macrophage presence? I'd rather not go > the biotin/streptavidin route that was suggested to me originally by a > vendor. > > Thanks in advance for any insight. > > David Polchert > Associate Research Specialist > Univeristy of Illinois-Chicago > ***************************EMAIL DISCLAIMER*************************** This > email and any files transmitted with it may be confidential and are intended > solely for the use of th individual or entity to whom they are addressed. > If you are not the intended recipient or the individual responsible for > delivering the e-mail to the intended recipient, any disclosure, copying, > distribution or any action taken or omitted to be taken in reliance on it, > is strictly prohibited. If you have received this e-mail in error, please > delete it and notify the sender or contact Health Information Management > 312.996.3941. >
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