Dear Ken, Are you sorting using Normal-R? Using Normal-R the "coincident abort events" are counted even when they are not sorted. If you see a very high abort frequency, this may be contributing to the discrepancy. Yours, Andrew --On Wednesday, November 27, 2002 17:41 -0500 "McDonald, Ken" <ken_mcdonald@Merck.com> wrote: > > Hi All, > I'm seeing a discrepancy in what the Vantage sorter tells me it's > sorted and the actual numbers sorted. For example, I sorted for green > calibite beads (30,000) and when I re-ran them to check for purity there > were roughly 15,000 only. Does anyone have the same problem? The purity is > good (99%) and everything else checks out by the book?..Thanks in > advance..Ken > > Ken McDonald (Biologist) > Merck Frosst Canada > Tel: 514-428-2802 > email: ken_mcdonald@merck.com > www.merckfrosstlab.ca > > > ------------------------------------------------------------------------- > ----- Notice: This e-mail message, together with any attachments, > contains information of Merck & Co., Inc. (Whitehouse Station, New > Jersey, USA) that may be confidential, proprietary copyrighted and/or > legally privileged, and is intended solely for the use of the individual > or entity named on this message. If you are not the intended recipient, > and have received this message in error, please immediately return this > by e-mail and then delete it. > > ========================================================================= > ===== > ---------------------- A Herman, Pathology & Microbiology A.Herman@bristol.ac.uk
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