The difference is very clear in fluorescence microscopy: FITC bleaches significantly faster than Alexa 488. We haven't compared the same antibody conjugated both ways, but among commercially-available secondary reagents, Alexa 488 conjugates seem a bit brighter in the flow cytometer too. Laird Bloom Phylos, Inc. 128 Spring St. Lexington, MA 02421 tel. (781) 862-6400 ext. 253 fax (781) 402-8813 www.phylos.com > ---------- > From: John Krowka > Sent: Thursday, March 21, 2002 11:58 AM > To: Cytometry Mailing List > Subject: FITC vs Alexa 488 > > > On Molecular Probes' website are claims that Alexa 488 is 6-7 times > brighter > than FITC. Preliminary evidence from our conjugations of the same antibody > with FITC or Alexa 488 indicate no advantage from using Alexa 488. Can > anyone who compared Alexa 488 and FITC share their findings? > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:04 EST