anti-mouse CD ab fail to react with wood mice blood cells

From: Van Bockstaele, Dirk (Dirk.Van.Bockstaele@uza.be)
Date: Thu Feb 28 2002 - 10:33:59 EST


Hi Flowers,

I recently got involved in a collaboration with scientists studying the
effect of environmental pollutants by investigating rodents (wood mice) they
catch in the field.
In preliminary experiments I was surprised to see that some monoclonals
against mice CD's (3/19/11b/13) that show normal reactivity towards lab mice
(Mus musculus) leukocytes fail to do so towards blood leukocytes of the wood
mice (apodemus sylvaticus).

My question: is this to be expected or not?  Is a lab mouse so different
from a wood mouse?  Or should I complain with the mab manufacturer and/or
start looking for another manufacturer?  I'm a complete novice regarding lab
mice work let alone wood mice!  Any hints, suggestions are very welcome!
thanks in advance.
Dirk

Prof. Dirk Van Bockstaele
Laboratory of Hematology
Head of Flow Cytometry / Molecular Diagnostics
Antwerp University Hospital
Wilrijkstraat 10
B-2650 Edegem
Belgium
phone 32 3 821 3900 fax 32 3 825 1148



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