Dear flow cytometry mailing list, We have published on an HIV-1 transgenic rat that has immunologic dysfunction. Recently we have shown that these rats also seem to have a Th1 deficit. I would like to isolate the naive (CD45RC+ and CD62L+) CD4 T-cell population by negative selection. Currently I can isolate the CD45RC+ population but this population is contaminated (20%) with CD62L- effector/memory T-cells. Does anyone know of available antibodies that can be used to remove the memory T-cell contamination from my CD45RC+ T-cell pool? You can contact me at 202-256-2984 or e-mail reid@umbi.umd.edu. Thank you for any help. Dr. William Reid Basic Science Division Institute of Human Virology University of Maryland Baltimore
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