We're trying to estimate the amount of DNA in the cells of a crustacean species by comparing the PI fluorescence of it to PI fluorescence of Drosophila cells. We are having problems both with the sample prep of drosophila (embryos or whole flies) and crustacean (we basically grind them up) and staining of the cells. Our major problem lies in the fact that we get so much debris that it is difficult to find populations of cells (or nuclei). If anyone has done anything similar to this, your experiences would be helpful and if anyone has specific experience measuring DNA in drosophila or crustacean-type species, that would be greatly appreciated also. We are using a BD LSR to analyze; 20ug/mL PI staining after detergent permeabilization (not fixed), and varying concentrations of cells (1x10^5 cells to 5x10^6 cells) Thank You, Ryan
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