Ebioscience (www.ebioscience.com) has rat-mouse Flk-1 (clone Avas12a1). I used this clone for flow cytometry and Western blotting. Jimin -----Original Message----- From: Jess Jankowsky [mailto:jankowsky.1@osu.edu] Sent: Wednesday, October 02, 2002 2:35 PM To: cyto-inbox Subject: mflk-1 antibody from Pharmingen I am trying to detect flk-1 on a mouse cell line. I am planning on using Pharmingen's rat anti-mouse flk-1 monoclonal antibody but am unsure what concentration will be optimal (unlabelled, follow by a 2ndary PE antibody) Has anyone ever used this antibody for flow and if so what concentration did you use? Jess Jankowsky Ohio State University jankowsky.1@osu.edu
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