Hello! I am a beginner for flow cytometry. I am working with NG-108 and PC-12 for apoptosis. I used Annexin V-FITC and PI for detection. However, I got diagonal pattern when plotting FL1 against FL3. Someone said that this is a auto-fluorescence pattern. So, do anyone experience the same auto-fluorescence problem with this two cell line? I removed the phenol red from the medium and tried auto-fluorescence compensation but the problem was still there. Do anyone have another method to solve this problem? Thanks! Raymond Department of Physiology The Chinese University of Hong Kong Hong Kong, China.
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