Dear Maciej, A good place to start is the Tetramer facility Guide from the NIAID/NIH http://www.niaid.nih.gov/reposit/tetramer/index.html <http://www.niaid.nih.gov/reposit/tetramer/index.html> You can also order a few tetramers at NIH if they are not commercially available through Coulter. Our own tetramer facility and research group published several interesting articles of which the most interesting for you is published in Blood 2002 Apr 1;99(7):2505-11, Persistent numbers of tetramer+ CD8(+) T cells, but loss of interferon-gamma+ HIV-specific T cells during progression to AIDS. Kostense S, Vandenberghe K, Joling J, Van Baarle D, Nanlohy N, Manting E, Miedema F. Department of Clinical Viro-Immunology, CLB/Sanquin Blood Supply Foundation, University of Amsterdam, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_ui ds=11895786&dopt=Abstract <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_u ids=11895786&dopt=Abstract> Our Tetramer facility group will be happy to help you start up. Best regards, John John Voorn Sanquin Reagents Product manager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: Maciej S. Simm [SMTP:mss2004@med.cornell.edu] Sent: Wednesday, August 14, 2002 5:54 PM To: Cytometry Mailing List Subject: Tetramers, p17, p24 Hello everyone, I've been asked to establish a protocol for detection of the HIV p17 and p24 specific T cells by tetramer staining. Does anyone have a good "starter" reference for the tetramer technique? as well as a source of purified p17? thanks for any input, Maciej
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:19 EST