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REAGENT SOPS

SOP#

TITLE  (BACK TO MAIN SOP)

Date Original 

Responsible person

P001

Preparation of 10x Hank’s Balanced Salt Solution

2/12/99

 

P002

Preparation of 10x Ammonium Chloride Lysing Reagent

2/12/99

 

P003

Preparation of Stock 1.1M Calcium Chloride

2/12/99

 

P004

Freezing HL60 Cells

2/12/99

 

P005

Operation Procedure for the Coulter Counter Model F/FN- White Blood Cell (WBC) Counts/ Red Blood Cell (RBC) Counts

2/18/99

 

P006

Preparation of 20mM DCFH-DA

2/12/99

 

P007

Preparation of 2 mM EDTA

1/12/99

 

            

Preparation of Working Hank’s Balanced Salt Solution (HBSS) with Calcium and Magnesium

2/15/99

 

P009

Preparation of Working Hank’s Balanced Salt Solution (HBSS) with Calcium, Magnesium, and 0.1% Bovine Serum Albumin (BSA)

2/15/99

 

P010

Preparation of Working Hank’s Balanced Salt Solution (HBSS) with Calcium, Magnesium, and 1% Bovine Serum Albumin (BSA)

2/15/99

 

P011

Preparation of Working Hank’s Balanced Salt Solution (HBSS) Without Calcium and Magnesium

2/15/99

 

P012

Preparation of Working Hank’s Balanced Salt Solution (HBSS) Without Calcium, Magnesium, with 0.1% Bovine Serum Albumin (BSA)

2/15/99

 

P013

Preparation of Working Hank’s Balanced Salt Solution (HBSS) Without Calcium, Magnesium, with 1% Bovine Serum Albumin (BSA)

2/15/99

 

P014

Preparation of 10mM Hydroethidine (HE)

2/15/99

 

P015

Heat Inactivation of Bovine Sera

2/15/99

 

P016

Preparation of Heparinized Vacutainer Tubes

2/15/99

 

P017

Preparation of Ammonium Chloride Lysing Reagent

2/15/99

 

P018

Preparation of Stock 0.4M Magnesium Sulfate

2/15/99

 

P019

Preparation of 2% Paraformaldehyde (PF)

2/15/99

 

P020

Preparation of Phosphate Buffered Saline (PBS)

2/15/99

 

P021

Preparation of Bleach-SDS

2/15/99

 

P022

Preparation of Working Medium Endothelial RPMI

2/15/99

 

P023

Calibration of the Orion Research EA 920 Expandable ionAnalyzer

2/15/99

 

P024

Determinaton of pH Using an Orion Research EA 920 Expandable ionAnalyzer

2/15/99

 

P025

Preparation of Working Medium RPMI 5/5

2/15/99

 

P026

Preparation of 1% Sodium Dodecyl Sulfate (SDS)

2/15/99

 

P027

Preparation of 10mg/ml Colchicine

2/15/99

 

P028

Preparation of Stock 1M TRIS

2/15/99

 

P029

Preparation of Stock a-MEM Medium

2/15/99

 

P030

Preparation of Working a-MEM Medium

2/15/99

 

P031

Dis-assembly of Coulter Counter for Cleaning and Replacing Internal Electrode

2/18/99

 

P032

Ficoll-Hypaque: Overlay Method

2/23/99

 

P033

Ficoll-Hypaque: Underlay Method

2/23/99

 

P034

Ammonium Chloride Lysis of Whole Blood

2/24/99

 

P035

Dextran Sedimentation

2/25/99

 

P036

Preparation of 3% Dextran

2/25/99

 

P037

CO2 /Nitrogen Tank- Replacing/ Re-ordering

2/26/99

 

P038

Operation of FYRITE CO2 Analyzer

4/19/99

 

P039

Operation of the AMSCO Autoclave

4/23/99

 

P040

Emergency Door Operation – AMSCO Autoclave

2/15/99

 

P041

Chicken RBC – Sterile Preparation for Flow Cytometric DNA Determination

4/30/99

 

P042

Operation Procedure for Coulter Counter-model Z2

1/4/01

 

P043

Operation Procedure for Orion Model 720A plus pH meter

2/19/01

 

P044

Preparing Biohazard Wastes for Autoclaving

6/12/02

 

P045

Operating the Biohazard Autoclave

6/12/02

 

P046

Preparation of  Nylon Mesh Filters

6/12/02

 

P047

Preparation of Modified Vindelov’s Propidium Iodide Stain

9/15/03

 

P048

Operation of PH meter - VWR model  9100

5/24/04

 

P049

Preparation of Grass Carp Fry for DNA Ploidy Screening

5/24/04

 

P050

How to Fill Out Bio-Materials Pick-Up Form

5/24/04

 

P051

Scanning of Optical Filters with USB 2000   (Word File)

7/30/04

 

P052

Shortlist- Operation of Coulter Counter model Z2

9/15/04

 

P053

Preparation of 0.1M HCl and 0.1 M NaOH Buffering Agent

8/30/04

 

P054

Decontaminating CO2 Incubator Using Paraformaldehyde

9/14/05

 

P055

Operation of Steam Sterilizer

1/6/06

 

P056

Operation of the Scanner

3/24/06

 

P057

How to Use ModfitLT Software

8/29/06

 

P058

How to Use a Counting Chamber

6/5/07

 

P059

Filter Scans

4/6/08

 

P060

Coulter Counter Z-Series “HOTPURGE” Procedure

4/10/08

 

P061

Cleaning Procedure for FC500 post Acridine Orange

5/8/08

 

P062

Pacific Blue Dilutions for HTS

8/17/09

 

P063

Point Source Fiber Optics – Laser Alignment

8/26/09

 

P064

PBS Gel Concentrate

1/11/10

 

P065

Amgen Screening Project – Dye Preparations (SEE ADDITIONAL SOPs at P104

3/1/10

 

P066

Hypercyte Data Analysis

2/11/10

 

P067

Making Agar for Bacteria Growth Rectangular plates (see P107 for Round Plates) (Bardot)

10/22/10

 

P068

Serial Dilutions for Bacteria (Bardot)

10/22/10

 

P069

Bimek/BacBuster Robot Procedure

4/4/11

 

P070

CyAn/HyperCyt/Biomek

4/4/11

 

P071

Beckman-Coulter Avanti-J-E Centrifuge Instructions for Use

4/28/11

 

P072

MoFlow XDP Start-UP Procedures

08-012011

 

P073

MoFlow XDP Shut-Down Procedures

8/2/11

 

P074

(Ray’s) Drip Plate_Serial Dilutions for bacteria

8/25/11

 

P075

Etest Strips_Antimicrobial Susceptibility Testing

8/30/11

 

P076

Standardizing the XDP for Cervical Cancer Sorting

1/30/12

 

P077

Check Laser Delay on CyAn

3/26/12

 

P078

Buffers for Cervical Cancer Study

5/22/12

 

P079

Cervical Cancer Cells Preparation

5/29/12

 

P080

Labeling Antibodies for Cervical Cancer Cells

5/29/12

 

P081

Amgen Automations HL60 - Ray

 

 

P082

Amgen Automations HL60 - Larisa

 

 

P083

XDP Total Power Shutdown

7/25/12

 

P084

XDP Sort Set-Up

7/26/12

 

P085

NZE Agar for Ecoli

9/19/12

 

P086

How to Determine MoFlo XDP  Laser Delay

1/17/13

 

P087

How to Store Bacteria in Glycerol for the -80 Freezer

2/27/14

 

P088

Thawing HL60 cells from the Cell Freezer

4/1/13

 

P089

Counting Cells using Nexcelom

4/1/13

 

P090

Feeding HL60 Cells

4/1/13

 

P091

Operation of the Beckman-Coulter MultiZier 4 (Bindley room 25)

4/5/13

 

P092

Start-up of the FC500 Gallios

5/22/13

 

P093

Shutdown of the FC500 Gallios

5/22/13

 

P094

BioMek 2000 – Compound Protocol

8/2/13

 

P095

Prepping tubes for testing Redox & JC1 Dyes for the Cyan.

 

 

P096

Setting up the Cyclone on the XDP MoFlo for a Sort

 

 

P097

Calibrating the BioMek 2000

9/23/13

 

P098

Bacteria in Ground Beef

10/30/13

 

P099

Nasty Bardot

2/10/14

 

P100

Upgraded XDP Start-up

 

 

P101

Upgraded XDP Shutdown

 

 

P102

Laser alignment upgraded MoFlo

 

 

P103

CytoFlex Start-up

 

 

P104

Cell Culture for Amgen HL60-old65a

 

 

P105

Flow Cyan Hypercyt Amgen-old65b

 

 

P106

Beads setting Cyan Amgen-old65c

 

 

P107

Making Bacterial plates (Round) -old P067

 

 

P108

 

 

 

P109

 

 

 

P110

 

 

 

P111

 

 

 

P112

 

 

 

P113

 

 

 

P114

 

 

 

P115

 

 

 

P116

 

 

 

P117

 

 

 

P118

 

 

 

P119

 

 

 

P120

 

 

 

P121

 

 

 

P122

 

 

 

P123

 

 

 

P124

 

 

 

P125

 

 

 

 

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