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REAGENT SOPS

SOP#

TITLE  (BACK TO MAIN SOP)

Date Original 

Responsible person

P001

Preparation of 10x Hank’s Balanced Salt Solution

2/12/99

 

P002

Preparation of 10x Ammonium Chloride Lysing Reagent

2/12/99

 

P003

Preparation of Stock 1.1M Calcium Chloride

2/12/99

 

P004

Freezing HL60 Cells

2/12/99

 

P005

Operation Procedure for the Coulter Counter Model F/FN- White Blood Cell (WBC) Counts/ Red Blood Cell (RBC) Counts

2/18/99

 

P006

Preparation of 20mM DCFH-DA

2/12/99

 

P007

Preparation of 2 mM EDTA

1/12/99

 

      P008     

Preparation of Working Hank’s Balanced Salt Solution (HBSS) with Calcium and Magnesium

2/15/99

 

P009

Preparation of Working Hank’s Balanced Salt Solution (HBSS) with Calcium, Magnesium, and 0.1% Bovine Serum Albumin (BSA)

2/15/99

 

P010

Preparation of Working Hank’s Balanced Salt Solution (HBSS) with Calcium, Magnesium, and 1% Bovine Serum Albumin (BSA)

2/15/99

 

P011

Preparation of Working Hank’s Balanced Salt Solution (HBSS) Without Calcium and Magnesium

2/15/99

 

P012

Preparation of Working Hank’s Balanced Salt Solution (HBSS) Without Calcium, Magnesium, with 0.1% Bovine Serum Albumin (BSA)

2/15/99

 

P013

Preparation of Working Hank’s Balanced Salt Solution (HBSS) Without Calcium, Magnesium, with 1% Bovine Serum Albumin (BSA)

2/15/99

 

P014

Preparation of 10mM Hydroethidine (HE)

2/15/99

 

P015

Heat Inactivation of Bovine Sera

2/15/99

 

P016

Preparation of Heparinized Vacutainer Tubes

2/15/99

 

P017

Preparation of Ammonium Chloride Lysing Reagent

2/15/99

 

P018

Preparation of Stock 0.4M Magnesium Sulfate

2/15/99

 

P019

Preparation of 2% Paraformaldehyde (PF)

2/15/99

 

P020

Preparation of Phosphate Buffered Saline (PBS)

2/15/99

 

P021

Preparation of Bleach-SDS

2/15/99

 

P022

Preparation of Working Medium Endothelial RPMI

2/15/99

 

P023

 Preparation of PBS gel conc and working dil'n

2/15/99

 

P024

How to make up dilutions for Pacific Blue dye for HTS

2/15/99

 

P025

Preparation of Working Medium RPMI 5/5

2/15/99

 

P026

Preparation of 1% Sodium Dodecyl Sulfate (SDS)

2/15/99

 

P027

Preparation of 10mg/ml Colchicine

2/15/99

 

P028

Preparation of Stock 1M TRIS

2/15/99

 

P029

Preparation of Stock a-MEM Medium

2/15/99

 

P030

Preparation of Working a-MEM Medium

2/15/99

 

P031

Dis-assembly of Coulter Counter for Cleaning and Replacing Internal Electrode

2/18/99

 

P032

Ficoll-Hypaque: Overlay Method

2/23/99

 

P033

Ficoll-Hypaque: Underlay Method

2/23/99

 

P034

Ammonium Chloride Lysis of Whole Blood

2/24/99

 

P035

Dextran Sedimentation

2/25/99

 

P036

Preparation of 3% Dextran

2/25/99

 

P037

CO2 /Nitrogen Tank- Replacing/ Re-ordering

2/26/99

 

P038

Feeding HL60 Cells

4/19/99

 

P039

Thawing HL60 cells from the Cell Freezer

4/23/99

 

P040

 

2/15/99

 

P041

Chicken RBC – Sterile Preparation for Flow Cytometric DNA Determination

4/30/99

 

P042

Amgen Automations HL60 - Larisa

1/4/01

 

P043

Amgen Automations HL60 - Ray

2/19/01

 

P044

Labeling Antibodies

6/12/02

 

P045

Preparing Cervical cancer cells

6/12/02

 

P046

Preparation of  Nylon Mesh Filters

6/12/02

 

P047

Preparation of Modified Vindelov’s Propidium Iodide Stain

9/15/03

 

P048

Buffers for Cervical Cancer Study

 

 

P049

Preparation of Grass Carp Fry for DNA Ploidy Screening

5/24/04

 

P050

How to Fill Out Bio-Materials Pick-Up Form

5/24/04

 

P051

Amgen Screening Project – Dye Preparations

 

 

P052

 

 

 

P053

Preparation of 0.1M HCl and 0.1 M NaOH Buffering Agent

8/30/04

 

P054

preparation of plasma samples for SPARC - TAN

9/14/05

 

P055

Preparation of heart blood plasma DiO rats SPARC Bob Philips

1/6/06

 

P056

Cell Culture for Amgen HL60

3/24/06

 

P057

Dye Prep for Amgen HL60

8/29/06

 

P058

Layout amgen-calcein-mbbr-mitosox PPT File

6/5/07

 

P059

 

 

 

P060

 

4/10/08

 

P061

 

5/8/08

 

P062

 

8/17/09

 

P063

 

8/26/09

 

P064

 

1/11/10

 

P065

 

3/1/10

 

P066

 

 

 

P067

 

10/22/10

 

P068

 

10/22/10

 

P069

 

 

 

P070

 

 

 

P071

 

 

 

P072

 

 

 

P073

 

 

 

P074

 

8/25/11

 

P075

 

8/30/11

 

P076

 

 

 

P077

 

 

 

P078

 y

5/22/12

 

P079

 

5/29/12

 

P080

 

5/29/12

 

P081

 

 

 

P082

 

 

 

P083

 

 

 

P084

 

 

 

P085

 

9/19/12

 

P086

 

 

 

P087

 

2/27/14

 

P088

 

4/1/13

 

P089

 

4/1/13

 

P090

 

4/1/13

 

P091

 

 

 

P092

 

 

 

P093

 

 

 

P094

 

 

 

P095

 

 

 

P096

 

 

 

P097

 

 

 

P098

 

10/30/13

 

P099

 

 

 

P100

 

 

 

P101

 

 

 

P102

 

 

 

P103

 

 

 

P104

 

 

 

P105

 

 

 

P106

 

 

 

P107

 

 

 

P108

 

 

 

P109

 

 

 

P110

 

 

 

P111

 

 

 

P112

 

 

 

P113

 

 

 

P114

 

 

 

P115

 

 

 

P116

 

 

 

P117

 

 

 

P118

 

 

 

P119

 

 

 

P120

 

 

 

P121

 

 

 

P122

 

 

 

P123

 

 

 

P124

 

 

 

P125

 

 

 

 

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