Many of these slides have been published in several of our Purdue Cytometry CDROM and DVD series, and may be used personally without copyright restriction provided you give credit to the author and reference this site. Commercial or for-profit use is strictly forbidden.
New EV detection technology www.celltient.com
Professors:
JPR: Dr. J. Paul Robinson, Purdue University Cytometry Laboratories
BR: Dr. Bartek Rajwa, PUCL
SK: Steve Kelley PUCL
WT: Dr. William Telford, Flow Lab at Hospital for Special Surgery, New York, NY
CCS: Dr. Carleton C. Stewart, (Retired)
SJS: Sigrid J. Stewart, (Retired)
BHD: Bruce Davis
Techniques for Giving a Great Seminar
This lecture will be helpful to students, postdocs and faculty and is focused on presenting that special seminar where you want to really communicate your message in a very professional way. This presentation shows you what to do and what not to do to get your message across.
Here is the Powerpoint file you can download.
Lectures:
#TitleProfSizeLink Presentation on CD4 low cost tests in AfricaJPR Listen Presentation 101 for Graduate StudentsJPR PageMicroscopy Series - BMS 524, "Introduction to Confocal Microscopy and Image Analysis."
1The Principles of Microscopy Part 1 [49 slides]JPR6.153 MBPPT2The Principles of Microscopy Part 2 [41 slides]JPR2.714 MBPPT3The Principles of Microscopy Part 3JPR PPT3aImage Formats and Image Manipulations [29 slides]SK5.794 MBPPT4aThe Principles of Microscopy Part 3 [31 slides]JPR4.265 MBPPT5Fluorescence and Fluorescent Probes [52 slides]JPR7.46 MBPPT6The Principles of Confocal Microscopy [44 slides]BR5.022 MBPPT73D Imaging, Rotations and Stereo Imaging [33 slides]JPR3.163 MBPPT8Principles of 2D image analysis - Morphometry (I) [46 slides]JPR1.623 MBPPT9Applications of Morphometry (II) [36 slides]JPR9.237 MBPPT10Live Cell Imaging Application in Confocal Microscopy [38 slides]JPR9.545 MBPPT11Applications of Confocal Microscopy [24 slides]JPR2.349 MBPPT12Sample Preparation of Confocal Microscopy [25 slides]JPR8.4 MBPPT13Additional Applications of Confocal Microscopy [25 slides]BR PPTFlow Cytometry Series - BMS 631, Slides are based on Howard Shaprio's great text "Practical Flow Cytometry" and many have page numbers to reference the material in that text.
AIntroductory flow cytometry: Part 1- E-lecture - automated online lectureJPR63 MinsListenBIntroductory flow cytometry: Part 2- E-lecture - automated online lectureJPR60 MinsListen Fluorescence Quantitation - An overview of the salient issues including QCJPR15.4 MBPPT1BMS 631 - LECTURE 1 Flow Cytometry: Theory (2006)JPR28.8 MBPPT2BMS 631 Flow Cytometry: Theory - Lecture 2 Whos and whys of flow cytometry - History(2006)JPR8.32 MBPPT3BMS 602A/631 - Lecture 3: : Light and Matter (2006)JPR0.68 MBPPT4BMS 602A/631: Lecture 4 : Light and Fluorescence (2005)JPR0.51 MBPPT5BMS 631 - Lecture 5: Properties and Applications of Light Sources (2005)JPR3.34 MBPPT6BMS 631 - Lecture 6: Optical Systems (2005)JPR24.53 MBPPT7BMS 631 - Lecture 7: Optics - Filter Properties and manipulation of light in flow cytometry (2005)JPR0.17 MBPPT8BMS 631: Lecture 8: Detectors (2004)JPR2.8 MBPPT BMS 631 - Lecture: Flow systems and hydrodynamics (2005)JPR8.41 MBPPT10BMS 631 - Lecture 10: Multiparameter data analysis (2004)JPR1.71 MBPPTThese lectures are undated, but many have been here for many years, so they may not be as up updated as you might like.
1Introduction to Flow Cytometry - 1 [28 slides]JPR5.3 MBPPT Page2Introduction to Flow Cytometry -2 [32 slides]JPR1.05 MBPPT Page3Flow Cytometry Lecture 3 - Light and Matter [28 slides]JPR213 KBPPT Page4Flow Cytometry lecture 4 - Optical Systems [29 slides]JPR1.94 MBPPT Page5Flow Cytometry Lecture 5 - Light Sources and Optical Systems [26 slides]JPR3.9 MBPPT Page6Flow Cytometry Lecture 6 - Optics: Filter Properties [41 slides] PageJPR235 KBPPT Page7Flow Cytometry Lecture 7 - Detectors & Fluidics [54 slides]JPR9.7 MBPPT Page8Flow cytometry lecture 8 - Electronic measurements & Signal Processing [24 slides]JPR95 KBPPT Page9Flow Cytometry Lecture 9 - Data Collection: Linear, Log, Ratios... [24 slides]JPR292 KBPPT PageOlder General Flow Cytometry Lectures
Med 101 [59 slides] (4/5/2005)JPR PPT Basic flow cytometry theory [17 slides]JPR Page DNA analysis [28 slides]JPR Page Multiple Color Analysis [3 slides]WT Page Light Scatter [2 slides]WT Page Excitation / Emission peaks [9 slides]WT Page Applications [14 slides]JPR Page Antibody Binding [49 slides]CCS Page Multiparameter Immunofluorescence [67 slides]CCS & SJS Page Compensation [16 slides]CCS & SJS Page Simultaneous Measurement of Necrotic, Apoptotic Dead and Live Cells [4 slides]CCS Page Immunophenotyping Leukemias & Lymphomas [23 slides]CCS Page Breast Cancer: DNA Content & Relevant Markers [4 slides]CCS Page Automated Reticulocyte Analysis [27 slides]BHD Page Immunophenotyping of Hematopoietic Progenitor Cells by Multiparameter Flow Cytometry [20 slides]CCS Page