BMS 524 Introduction to Confocal Microscopy and Image Analysis -2016

This page is for the use of students who are taking

BMS 524 - 2016 Notes - Vet Path Research Bldg 119

Professor J. Paul Robinson, Purdue University (Email me at jpr(at)


NOTE: The use of these materials is restricted without specific permission of Professor J. Paul Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. While fair use by not-for-profit institutions is allowed, appropriate acknowledgement is requested and should be included on every slide used.


BMS 524 final is not yet set. There will be a midterm exam and a final exam.


2016 Lecture Schedule


BMS 524 – 15 lectures

Date Lect #Title LectPPT File (Download) View the Lecture
Jan 131The Principles of Microscopy (1)JPR

30 megs

126 meg (2014)


2016 Lect

2012 lecture

Video lecture (2009)

Jan 202Principles of Microscopy (2)JPR

3.3 megs

100 meg (2014)


2016 lect

updated 2016
Jan 273Principles of Microscopy (3)JPR

5.3 megs

112 meg recorded lecture


2016 recorded Lecture

Video lecture (from 2009)

NEW VIDEO Lecture from


Feb 34Image Formats and Image ManipulationsJPR  2016 lecture 
Feb105Fluorescence and Fluorescence Probes xxx5.0 megs
updated 2016
Feb176Principles of 2D digital image analysis - (I)JPRLecture 6updated 2016
Feb 247Principles of 2D digital image analysis (II) JPRLecture 7updated 2016
March 2 

Midterm quiz (take home)

Components of the microscope

JPRLecture 8Updated 2016
Mar 98Sample Preparation of Confocal MicroscopyJPR2.3 meg PDFUpdated 2016
Mar 16 


Spring Break

Mar 239Imaging at Purdue- STED, STORM and BR




Mar 3010



Novel 3-D microscopy techniques: 2-photon, 4Pi

BR12 megs 
Apr 611

Live Cell Imaging Applications in Confocal Microscopy






Updated 2016
Apr 1312

Image Presention, Manipulation & Fraud

JPR Updated 2016
April 2013

Spectral Imaging Lecture


BR2.6 megs downloadUpdated 2016
April 27 




You can click on the links to download or directly view the Powerpoint files. NOTE: most of these lectures are relatively large.

JPR (Dr. J. Paul Robinson), BR (Dr. Bartek Rajwa)


NIH Electronic Directory
NSF Award Search
PubMed Central
NIH Reporter