This page is for the use of students who are taking

BMS 524 - 2008 Notes

Introduction to Confocal Microscopy and Image Analysis

Professor J. Paul Robinson, Purdue University (Email me at jpr#flowcyt.cyto.purdue.edu)

The final exam date for 2008: Tuesday April 29th from 10:30 - 12:20 in VPTH 112

If this is a problem for anyone please see Professor Robinson for an alternative date.

NOTE: This not a public access page and the use of these materials is restricted without specific permission of Professor J. Paul Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. While fair use by not-for-profit institutions is allowed, appropriate acknowledgement is requested.

If you don't see a pop-up window with the BMS 524 Class Survey and Practical Information Form please click here to take the survey. If you do not take the survey, you will not be considered for the practical course. Send the survey to jennie@flowcyt.cyto.purdue.edu

You can click on the links to download or directly view the Powerpoint files. NOTE: most of these lectures are relatively large.

2008 Lecture Schedule

BMS 524 – 15 lectures

NOTE: Video lectures will only be posted on a weekly basis.

Lecturers: JPR: J.Paul Robinson; BR: Bartek Rajwa, SK: Steve Kelley, JI: Joseph Irudayaraj; SL Dr. Silas Leavesley

• Lecture 1 - 1/10/07:The Principles of Microscopy (1) (23 megs) (JPR)
• Lecture 2 - 1/17/07: Principles of Microscopy (2) (2.8 megs) (JPR)
• Lecture 3 - 1/24/07:Image Formats and Image Manipulations (5.5 megs) (SK)
• Lecture 4 - 1/31/07:Principles of Microscopy (3) ( 5.2 megs) (JPR) View the Video lecture (OK)
• Lecture 5 - 2/7/07:Fluorescence and Fluorescence Probes (7.7 meg) (JPR View the Video (OK)
• Lecture 6 - 2/14/07:Principles of 2D image analysis - Morphometry(I) (JPR) View the video (*)
• Lecture 7 - 2/21/07:Applications of Morphometry (II) (JPR) View the video lecture (*)
• Lecture 8 - 2/28/07:The Principles of Confocal Microscopy (19.2 megs) (BR) View the video (*)
• Lecture 9 - 3/7/07:Novel 3-D microscopy techniques: 2-photon, 4Pi... (BR) (OK)

SPRING BREAK - 3/14/07 - No Class

• Lecture 10 - 3/21/07:Applications of Confocal Microscopy (JPR)
• Lecture 11 - 3/28/07: Sample Preparation Techniques (JPR) 8megs - 3/28/07 View the video (OK)
• Lecture 12 - 4/4/07: Intrinsic optical properties of cells: autofluorescence, albedo, SHG (BR)
• Lecture 13 - 4/11/07: TBA
• Lecture 14 - 4/18/07: "Raman Confocal microscopy: Principles and Applications (JI)
• Lecture 15 - 4/25/07: Spectral Imaging and Review (JPR)

Lecturers: JPR: J.Paul Robinson; BR: Bartek Rajwa, SK: Steve Kelley, JI: Joseph Irudayaraj

These notes are not for publication or distribution without the written permission of Professor Robinson