BMS 631 Flow Cytometry: Theory (2 CR Course) - 2022 Course Notes

This page is for the use of students who are taking BMS 631

2022 Notes - Location: Vet Path Research Bldg 119

This is a 2 credit course that is designed to bring a student up to date on the technology of flow cytometry. The course will be lecture based and will require students to give a short seminar at the conclusion of the semester which will be part of the final grade. The course starts with the historical discovery of the technology and will move to discussing each of the individual components - optics, electronics, fluidics, data processing and analysis. In addition, we will cover all of the current applications. At the conclusion of the course, students will have an excellent understanding of the field and will be capable of reading, interpreting and carrying out studies using this technology.

Purdue University Cytometry Laboratories

NOTE: The distribution of these materials is restricted without specific permission of Professor Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically, no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions and permission should be requested and credit included when used in this instance. We normally give permission for use of these materials in academic institutions.If you submit these to course hero, course hero has been notified that anyone submitting these lectures must be reported back to Purdue University.


Class text is "Practical Flow Cytometry", 4th ed (Shapiro)  you can download a free copy here or buy on ebay/Amazon

Here is the Introduction to Watson's Book - worth a read

Date2022 Lect #Older lecturesLectFile (incl older lectures)File Size Link
Jan 10

2022 PPT


Lect 1 Video

Lect 1 2022


2020 PPT lect1

2020 lext 1 PDF


Introduction, history & basic flow principles (Pt 1) (2019)




(Automated PPT) 2016 Lecture

Jan 24

Lect 2 PPT


Lect 2 Video

Lect 2 2022


The Who and Why of Flow Cytometry: History Cont. (Pt 2) (2020 Version)

Recorded On Line class 27 Jan



NEW Video lect

from Jan 25, 2016






Jan 243

Light, Matter, and the basics of scatter 

2020 Lecture

Recorded 2020 lecture is here.


Jan 31





2020 Lecture 4







Feb 146

Light Sources


2020 Lecture

JPRLecture 6  
Feb 21

7 & 8


Properties of Optical Filters


- Detecton Systems 2019




Feb 289 & 10

Fluidic Systems 2019


2020 Fluidics lecture


2020 Video Recording Lect8



Data structures




Lecture 10



Mar 7


Cell function 

+ Lecture 12 DNA-RNA cytometry

Mar14-19Spring Break






Microbiolgy & Cytometry


2020 PPT


2020 online Lecture





Mar 28


2020 Cell function PPT


2020 Online lecture



Lecture 13.ppt + Lecture 14.ppt



Apr 18


2019 Cell Sorting


2020 Cell Sorting PPT


2020 Online Sorting


2020 Spectral flow cytometry


2020 Spectral online lect


April 25

Class Presentations

We will schedule these on webex


Class Presentations

On line webex   
Final Exam Take homeJPR   

Lecturer: JPR: J. Paul Robinson (contact by email:



Seminars should be 10-15 minutes and must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.


NameDateExample Topics (must be related to flow cytometry and cannot be your research project - some examples are below)
  Fluorescent probes for Oxidative Metabolism
  Monoclonal antibodies, Avidin-Biotin technology using Fluorescent Conjugates in Flow cytometry
  Principles of Sorting in Flow Cytometry.
  Intracellular cytokine determination via flow cytometry
  Optical Filters used in Flow Cytometry
  Cell proliferation assessed by flow cytometry using cell tracking dyes
  Data analysis: How and why?
  Fluidics Systems in Flow Cytometry
  The principles of spectal overlap
  Parameters measured in Flow and what they mean
  Fluorescent probes for apoptosis


All materials copyright © J. Paul Robinson 1989-2022 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions and credits must be included for all slides used. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.


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