This page is for the use of students who are taking

BMS 631 Flow Cytometry: Theory (2 CR Course)

Purdue University Cytometry Laboratories

Final Exam: Tuesday April 29 3:20 to 5:20 in Lynn G167: As usual, if you have a conflict with another final email me at jpr#flowcyt.cyto.purdue.edu

NOTE: This not a public access page and the use of these materials is restricted without specific permission of Professorl Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions. We normally give permission for use of these materials in academic institutions.

Also, please note that I have added a couple of e-lectures that highlight the introduction to flow cytometry. These are in the E-Lectures option one level previous to this page.

E-lectures (You can take a look at these lectures - they are designed as introductory lectures for those will no knowledge of flow cytometry.)

• To download the Powerpoint file, you can click and save or just run the file from the server.
• 2008 Class notes and lecture Scedule - BMS 631: Flow Cytometry: Theory

Lecturer: JPR: J. Paul Robinson (contact by email: jpr#flowcyt.cyto.purdue.edu)

2008 Student Seminar Schedule: We will determine seminar dates soon.

Seminars must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.

2006 Lecture Schedue - I will leave these lectures here until the end of the semester. 2008 lectures will be updated from these.

• Lecture 1: Introduction, history Basic Flow Principles (28,041 KB)
• Lecture 2: Whos and Whys of Flow Cytometry: History Continued (1,137 KB)
• Lecture 3:Light and Matter (715 Kb)
• Lecture 4:Light and Fluorescence (479 Kb)
• Lecture 5:Properties and Applications of Light Sources (3.33 megs)
• Lecture 6:Optical Systems in Flow Cytometers (22 megs)
• Lecture 7:Optics - Properties of Optical Filters (9.76 megs)
• Lecture 8:Detectors (2.815 megs)
• Lecture 9:Fluidic Systems (8.376 megs)
• Lecture 10: Multiparameter Data Analysis (2.071 megs)
• Lecture 11: DNA-RNA probes (773 kb)
• Lecture 12: Cell Function (2.505 megs)
• Lecture 13: Microbiology (2.313 Megs)
• lecture 14: Student Seminars
• Lecture 15: Student Seminars

All materials copyright © J. Paul Robinson 1989-2008 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.