BMS 631 Flow Cytometry: Theory (2 CR Course) - 2020 Course Notes

This page is for the use of students who are taking BMS 631

2020 Notes - Location: Vet Path Research Bldg 119

This is a 2 credit course that is designed to bring a student up to date on the technology of flow cytometry. The course will be lecture based and will require students to give a short seminar at the conclusion of the semester which will be part of the final grade. The course starts with the historical discovery of the technology and will move to discussing each of the individual components - optics, electronics, fluidics, data processing and analysis. In addition, we will cover all of the current applications. At the conclusion of the course, students will have an excellent understanding of the field and will be capable of reading, interpreting and carrying out studies using this technology.


Purdue University Cytometry Laboratories

NOTE: The distribution of these materials is restricted without specific permission of Professor Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically, no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions and permission should be requested and credit included when used in this instance. We normally give permission for use of these materials in academic institutions.If you submit these to course hero, course hero has been notified that anyone submitting these lectures must be reported back to Purdue University.


Also, please note that I have added a couple of e-lectures that highlight the introduction to flow cytometry. These are in the E-Lectures option one level previous to this page.


E-lectures (You can take a look at these lectures - they are designed as introductory lectures for those will no knowledge of flow cytometry.)


Class text is "Practical Flow Cytometry", 4th ed (Shapiro)  you can download a free copy here

DateLect #2020 LecturesLectFile (incl older lectures)File Size Link
Jan 131

2020 PPT lect1

2020 lext 1 PDF


Introduction, history & basic flow principles (Pt 1) (2019)




(Automated PPT) 2016 Lecture

 updated 2020
Jan 222The Who and Why of Flow Cytometry: History Cont. (Pt 2) JPR


NEW Video lect

from Jan 25, 2016

 updated 2020

Jan 27

3ON Line Class 


Webex link in email

 Updated 2018
 3Light, Matter, and the basics of scatter JPRLecture003.ppt updated 2018













Feb 105

Optical Systems



Lecture  Video NEW Link to recording 2016

Lecture 5 link

 updated 2016
Feb 176Light SourcesJPRLecture 6 Updated 2018
Feb 24

7 & 8


Properties of Optical Filters


- Detecton Systems 2019




 updated 2018
Mar 29 & 10

Fluidic Systems 2019 &

Data structures




Lecture 10

 updated 2018




Cell function 

+ Lecture 12 DNA-RNA cytometry

Mar 16








No Lecture




 Updated 2018

Mar 30



Microbiolgy & Cytometry



Lecture 13.ppt + Lecture 14.ppt



Apr 6webex lectureCell SortingJPRWebex link by email  
April 13Class Presentations     
Final Exam Take homeJPR   

Lecturer: JPR: J. Paul Robinson (contact by email:



Seminars should be 10-15 minutes and must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.


NameDateExample Topics (must be related to flow cytometry and cannot be your research project - some examples are below)
  Fluorescent probes for Oxidative Metabolism
  Monoclonal antibodies, Avidin-Biotin technology using Fluorescent Conjugates in Flow cytometry
  Principles of Sorting in Flow Cytometry.
  Intracellular cytokine determination via flow cytometry
  Optical Filters used in Flow Cytometry
  Cell proliferation assessed by flow cytometry using cell tracking dyes
  Data analysis: How and why?


All materials copyright © J. Paul Robinson 1989-2019 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions and credits must be included for all slides used. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.


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