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BMS 631 Flow Cytometry: Theory (2 CR Course) - 2024 Course Notes

This page is for the use of students who are taking BMS 631

2024 Notes - Location: Vet Path Research Bldg 119

This is a 2 credit course that is designed to bring a student up to date on the technology of flow cytometry. The course will be lecture based and will require students to give a short seminar at the conclusion of the semester which will be part of the final grade. The course starts with the historical discovery of the technology and will move to discussing each of the individual components - optics, electronics, fluidics, data processing and analysis. In addition, we will cover all of the current applications. At the conclusion of the course, students will have an excellent understanding of the field and will be capable of reading, interpreting and carrying out studies using this technology.

Purdue University Cytometry Laboratories

NOTE: The distribution of these materials is restricted without specific permission of Professor Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically, no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions and permission should be requested and credit included when used in this instance. We normally give permission for use of these materials in academic institutions.If you submit these to course hero, course hero has been notified that anyone submitting these lectures must be reported back to Purdue University.

 

Class text is "Practical Flow Cytometry", 4th ed (Shapiro)  you can download a free copy here or buy on ebay/Amazon

Here is an outline of the topics in the course.

Here is the Introduction to Watson's Book - worth a read

Date

 

Older lecturesLectFile (incl older lectures)File Size Link
Jan 8

2024 Lect #1a&b PPT

 Video Pt 1

 

Note: Jan 15 is MLK day.

2023 Lect 1

2023 1 Video

2020 PPT lect1

2020 lext 1 PDF      

 

Intro history &flow principPt12019

JPR

 

  
Jan 22

Concluding Lecture 1

 

 

2023 2 PPT

2023 2 Video

Lect 2 2022

Who,Why of Flow Cytometry (Pt 2-2020 On Line class

JPR

 

  

 

   

 

 

  
Jan 29

Recorded lecture

only today Lect 002- Jan 29

 

Here is the PPT

2023-3PPT

2023-3Video

Light, Matter, and the basics of scatter 

2020 Lecture

Recorded 2020 lecture is here.

JPR   

Feb 5

 

 

Online lecture only today Lect 3

(Light and matter)

 

Here is the PPT

 

2023-4PPT

2023-4 video

Fluorescence

2020 Lecture 4

JPR

 

 

 

Feb 12

Here is the PPT

Video -Pt 1

Video - Pt 2

Light Sources

 

2020 Lecture

JPR   
Feb 19

 

Recorded lecture only today 

Here is the PPT

 

2024 - Video Optical Systems

 

Optical Filters 2023 Video From Sl27 only

2023-6 PPT

2023-6Video

Properties of Optical Filters

- Detecton Systems 2019

JPR

 

  
Mar 4 

 

Lect PPT

Video Lect 

 

Optical Filters

 

2023-7PPT

2023- 7Video

 

2019 Fluidic Systems 

2020 Fluidics lecture

2020 Video

JPR

 

  

 

Mar 11

Spring break

Cell function 

+ Lecture 12 DNA-RNA cytometry

JPR   

March 18

Lect 7 -PPT

Light Sources

Fluidics

Lect 8 PPT

 

 

Lect8Video2023

Fluidic systems

2023  Video here2023

    

Mar25

Video

ONLY

ONLINE LECTURE ONLY

 

Lect9PPT

Lect9Video

 

Microbiolgy & Cytometry

 

2020 PPT

 

2020 online

 

 

 

  

April 1

 

Data structure

Lect10PPT

 

Lect10Video2023

2020 Cell function PPT

 

2020 Video lecture

JPR

 

 

 

Apr 8

 

Cell Sorting

Lect11PPT

 

Lect11Video 2023

2019 Cell Sorting

2020 Cell Sorting PPT

2020 Online Sorting

2020 Spectral flow cytometry

2020 Spectral online lect

JPR   
April 15

class Presentations

 

    
April 24

No class

    
       
May 4 end term      
Final Exam Take homeJPR   

Lecturer: JPR: J. Paul Robinson (contact by email: jpr#flowcyt.cyto.purdue.edu)

 

 

Seminars should be 10-15 minutes and must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.

 

NameDateExample Topics (must be related to flow cytometry and cannot be your research project - some examples are below)
  Fluorescent probes for Oxidative Metabolism
SD03/15Monoclonal antibodies, Avidin-Biotin technology using Fluorescent Conjugates in Flow cytometry
  Principles of Sorting in Flow Cytometry.
XW03/15Intracellular cytokine determination via flow cytometry
SH03.19Optical Filters used in Flow Cytometry
KK03/15Cell proliferation assessed by flow cytometry using cell tracking dyes
PH03/16Data analysis: How and why?
  Fluidics Systems in Flow Cytometry
  The principles of spectal overlap
RU03/16Cytof- Mass Cytometry
CL03/15Fluorescent probes for apoptosis
PS03/16Parameters and probes for evaluation of DNA &RNA in Flow Cytometry

 

All materials copyright © J. Paul Robinson 1989-2024 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions and credits must be included for all slides used. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.

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