Confocal Microscopy and Image Analysis

Image Analysis and Software Capabilities

Currently available image processing tools

Computers:

• Silicon Graphics Workstation
• IBM IntelliStation M Pro
• Sony Vaio computers
• Dell PowerEdge 1800 (controls MRC1024 system)
• Dell Dimesion (controls 2100 MP system)

Software packages:

• Image-Pro Plus from Media Cybernetics
  • 3D Constructor plug-in
  • Sharp Stack plug-in
• MetaMorph and MetaFluor from Universal Imaging
• Adobe Photoshop with Image Processing Toolkit
• VoxelView

The following image analysis programs were installed in the lab in 1994 and were available to users

The following materials is retained for historical purposes only (1994-2002 site materials)

Silicon Graphics (2 Indigo 2 Workstations):

• Vital Images VoxelView Software (pretty complicated program, powerful, not exactly intuitive to use)

IBM PC Compatible Systems (Compaq Prosignia 500, 80 MB RAM):

• MS DOS
  • COMOS (can run the confocal scope)
• Running Under OS2 Warp
  • PM View (fantastic shareware program - a MUST if you have OS2)
  • PM Jpeg (same comment as above)
  • LASERSHARP 1024 - 1024x1024 - software for running the MRC 1024
• Running Under Windows
  • OPTIMAS (OK - useful features, but limited - the good stuff is usually in the next upgrade!)
  • Adobe Photoshop
  • Microvoxel (OK - could take it or leave it - has some useful features)
  • Microsoft Powerpoint (slides)
  • Deltagraph (Good for graphs - OK for slides)
  • Bravo (partially useful - not as friendly as some!)
  • PRISM v.2, Graphpad Software (Excellent for graphs and stats)
  • Confocal Assistant (very useful)
  • PStyler (very useful for quick manipulation of images)
  • Corel Draw (Clever program, OK, only use when need too)
  • Metamorph/Metafluor, Universal Imaging (Expensive, but pretty good software)