“Not only do I stand on the shoulders of the giants, but
also I try my best to provide my shoulders for others to
stand on.”
--Peng Xi, Purdue University Cytometry Labs
PIC
Converter
You might have experienced the power of
Confocal Assistant.
Now, when you have a set of images which is NOT Bio-Rad PIC
format, what can you do to use Confocal Assistant? I
searched hard and found that there is no simple answer. In
this website Lance Ladic gave a solution to this (http://www.biotech.ufl.edu/EM/data/tiff.html),
but it is difficult to put into practice. So, I
compiled this small program to answer this question. In
order to recognize the various image formats, I
used
NIH ImageJ, a very professional open-source image
processing software presented by Wayne Rasband.
Usage: Use
ImageJ to convert your image sequences to RAW data
series, then use PIC converter to convert the raw files to
Bio-rad PIC format.
Step
1: Open ImageJ, File-Import-Image Sequences;
Step
2: Convert all images to 8-bit. Choose Image-type-8
bit.
Step 3: Save the image sequences to
Raw data series. Choose File-Save As-Image Sequences,
then select “Raw” and name the data series.
Step
4: Load the image files in PIC Converter. Use Control or
Shift key to select multiple files as shown in the red
circle.
Step
5: Name the destination file, and input the width and
height pixel number of the image (shown in the green
circle). Click “Convert” to start the
conversion. The destination PIC file
will be stored on the same working path as the raw data.
Step
6: If you like this small software, send an email to the
author! It is extremely easy: Click on Help-About-Email
author, an Email will be prepared for you to send! Your
support is very important to the author.
Download available for: Windows
95/98/NT/2000/XP
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