This is not a general distribution site. When you open this page and download the software, you are agreeing not to reverse-engineer or incorporate this software into any commercial product.
If you use Cytospec, Please acknowledge it in the following way: Cytospec Software is from Purdue University Cytometry Laboratories (and if you wish you can put the link (http://www.cyto.purdue.edu/Purdue_software)
LDATAPP - Convert FCS files to ASCI
(Written by Steve Kelly at Purdue University Cytometry Labs)
|Contributor: Purdue University Cytometry Laboratories|
|Authors: Valery Patsekin, Steve Kelley, Gerald Gregori, J. Paul Robinson|
|LDATAPP dumps FCS listmode or histogram data into ASCII text files. It has a simple interface to select input files and output paths. It requires a 32 bit Microsoft Windows operating system.|
(The help function was changed in more recent versions of Windows and you must download a special conversion file from Windows website to make the help work in Windows 7 or higher
CYTOSPECTM (updated Dec 6, 2013)
Version 22.214.171.124 (why so many OHs!! ) is now updated with protocol saving. The following program is called Cytospec(TM) and is a general purpose flow cytometry analysis program
Brief instructions for running Cytospec are here (download now) and will be updated one of these days!
1. Tutorial without audio (use above file) (play)
The following video tutorials teach you all the basics of Cytospec. You can use the 5 color listmode file HERE if you want to play along!
Note: this is not currently uploaded to this site.
Download requires specific request to J.Paul Robinson - send me an email with you name and institution and a request to jpr(at)flowcyt.cyto.purdue.edu - Note: while this is free to not for profit institutions, corporations who wish to use this software require a reseach and development contract with PUCL. This ensures our group can support your needs.
We frequently request a data set from you to test the program prior to giving you the software. This is to ensure that your files will run on this software.
PlateAnalyzerTM is a program for high content flow cytometry analysis. It requires specific license document which will be prepared for each user. In order to do this, you must send an email to jpr(at)flowcyt.cyto.purdue.edu and request a copy providing your name, and institution. (NPF only)
Drug concentration file (This is an example, but you can make any one you want) (download now)
Sample Data file: zip file - this must be unszipped - it contains 384 well data - each are fcs files showing 32 drugs (30 megs)
Tutorial files (these were made on an earlier version, but they are very similar to the current version) Many of these are on YouTube - search for "PUCL, PlateAnalzyer, Purdue"
3. Basic analysis (download now) (23.8 megs)
8. AutoUpdate feature (play) auto%20updates.wmv
10. SMILES codes read for drugs see YOUTUBE
11. New DRUGBOX Controls features YOUTUBE
12. Export CSV data files YOUTUBE
13. Dealing with Empty Wells YOUTUBE
14. 40 PLUS parameter data CyTOF analysis YOUTUBE
15. Autowell finder-extracting single wells from large files YOUTUBE