This demo is an example of an 11 color flow cytometry experiment.
You can explore the incredible heterogeneity of the T cell
compartment in man by gating on any of these subsets and viewing
the expression several additional markers. Explore what the
expression of CD28, CD57, CD49f are on these differentiation
and lineage subsets... see if you can determine how many subsets
of T cells are revealed by this staining! (Hint: at least
100).
The PBMC sample in this collection was stained with 11 different
antibodies: FITC anti-CD28, PE anti-CD49f, Cy5PE anti-CD11a,
Alexa495, anti-CD62L, APC anti-CD45RO, Cy7APC anti-CD57, Cy7PE
anti-CD4, Cascade Blue anti-CD45RA, Cascade Yellow anti-CD3,
Cy5.5PE anti-CD27, and Cy5.5APC anti-CD8.
Files:
| File |
Description |
| 11color.hqx |
BinHex file containing
a StuffIt archive with FlowJo, its workspace file,
more explanation, and data in a FlowJo specific version.
Macintosh only. |
| 11color.fcs |
A standard FCS file
for viewing the data with other analysis programs.
Platform independent. |
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For
more information contact:
J.
Paul Robinson, Ph.D., Director PUCL, Hansen Hall, B050, Purdue
University, West Lafayette, IN 47907, USA Phone: (765)494-0757,
Email to jpr@flowcyt.cyto.purdue.edu |
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