PUCL Job#1317 Res Asst - Mem Sloan Kettering NY (05032021)

Memorial Sloan Kettering Cancer Center

Immune Monitoring Facility

New York, NY


 Job Description Summary

Perform out a variety of laboratory procedures in support of immune monitoring of cancer immunotherapy trials, mainly focused on developing and validating multicolor flow cytometry assays on the BD LSR Fortessa X-20 and FACSymphony X-50 (antibody panel development for surface and intracellular staining), clinical sample testing with validated flow assays, and some cellular culture work (whole blood PBMC isolation, stimulation and culturing of human cells). Experience with high dimensional multi-parameter flow analysis using data reduction and biostatistics tools would be valuable. Experience with other types of immunology assays (ELISA, multiplex immunoassays) would be a plus but not required.

Job Description

We are currently searching for a Research Assistant to support the Immune Monitoring Core Facility in the Ludwig Center for Cancer Immunotherapy at Memorial Sloan-Kettering Cancer Center.  Responsibilities for this position include:

·       Developing and performing a wide variety of complex procedures and techniques which may include, but are not limited to: multiparameter flow cytometry assays (primary expertise, surface and intracellular staining methods plus high dimensional flow data analysis), single and multiplex immunoassays, PBMC isolation/cryopreservation from whole blood, tumor tissue processing, and assistance in the accurate maintenance of clinical sample inventories for proper sample tracking.  

·       Researching scientific literature for methods and procedures that may be considered for application or modification to current procedures; optimizing/troubleshooting methodologies where appropriate; ordering and maintaining laboratory supplies as needed for experiments.

·       Drafting and strictly adhering to laboratory standard operating procedures for assays and routine methodologies; carrying out assay validation experiments; drafting technical reports; maintaining accurate, daily records of experiments, patient sample information, material/reagent lots, and results in laboratory notebooks.

·       Analyzing data and creating graphs using appropriate software (e.g., FlowJo, Prism) and data visualization tools; writing reports of results and methods used; interpreting and discussing results of each experiment with supervisor as part of the planning process for subsequent studies; presenting data at regular lab meetings.

·       Routine maintenance and operation of a variety of laboratory instruments used to perform sample analyses which may include, but are not limited to: multicolor flow cytometers, ELISA readers, multiplex protein analyzers.

·       Following proper safety recommendations and handling procedures for different biological and chemical materials in regular use in the laboratory.

·       Training other laboratory personnel as needed in the use of sophisticated laboratory equipment and in the performance of specialized procedures or techniques.

·       Performing all other duties as assigned.


Please send resume to: Dr. Phillip Wong (


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