Fredrik Wallberg

Last Updated: 4 Nov 2013 (Check what has been updated)

Version as of November 2013 is 3.9 but it may be a little unstable as some new features are not verified.

Please download the following files.

I suggest you play the tutorials while playing with the files. This way you can follow along with the tutorial. There are more basic tutorials at our EDUCATION page, Tutorials. They may be helpful.

PlateAnalyzer Programs - there are three files - the main file is platAn.exe, there is an INI file which records your file history (and is only created when you first run the program), and your license file. All three files must be in the same directory. The INI file records your preferences, you past analysis and protocol lists and the layout of your screen. If you have serious problems with the software, you can delete the INI file and it will reset the software to default. You will only loose the history file - which you can easily recreate. The files are coded for your use only. You may use as many copies as you want for your lab use, on as many computers as you want, but they will all be coded with your name. If you want a collaborator to have a copy please send JPR an email.

Download the parameters and plate layout files and I suggest you create a directory to identify them as such.

Some Demonstration TUTORIALS for PlateAnalyzer

New Tutorial for your LSRII Data

1. PI Positive VS PI negative (right click to download 7 meg)

Go to Tutorials

Full Plate Examples for Drug Dose Responses

Example plate 58 - 384 well plate with JC-1 (ZIP file unzip to use)
Example plate 65 - 384 well plate with Redox (Zip file unzip to use)

Parameter & Plate Layout

Example drug concentration CSV file (use for plates 58 & 65)

Example PAP file for Plate 58 (simple layout)

Example PAP file for Plate 58 (advanced layout)

Drug Concentration File (CSV) for 58 and 65

Example PAP file for Plate 65 (advanced layout)

Example PAP file for Plate 65 (Very advanced layout) this layout shows how you can create an entire report in a single protocol to produce all 96 graphs.This is pretty cool!

Parameter & Plate Layout for Cytof

Vertical Drug Concs. CSV file

Vertical Drug Concs (reversed!!) checking if this is correct!!

Example complex layout for Sunitinib data set (works for others)

More compelex file as on 11/29 call - Sunitinib data set (ditto)

Sunitinib data file DE-BARCODED (418meg zip)

New Logic Map Protocol for March 8 2-12 with New DRUGBOXES

Tutorials (mainly for Cytof users)

1. Initial Ideas (Sept 26, 2010)

2. Here are the general tutorials

3. Specific tutorial for Bodenmiller data only (37 meg)

4. Decoding Tutorial is here (4.6 meg)

5. My Video of Checker (2 dec) (5meg)

6. My Video of Starsnstripes (2 dec - low audio sorry!) (7meg)

7. March 8, 2012 - New Drugbox controls

(There are additional CYTOF files available)

Data Files

Programs

32 bit:

PlatAn.exe (the latest version of the main program)
PlatAn.ini (can be deleted any time but it remembers what you have done - if you have major problems and the program seems possessed, just deleted this file and start again). The main program installation is in c:/program files (x86)/PUCL/Plate Analyzer (x86 for 64 bit) and c/:program files/PUCL/Plate Analyzer (for 32bit computers). The download is to your /users/name directory (windows 7 does not allow editing files in main program directory) but the program is run from the c:/program files directory and this is where you MUST place your LIC file.

CyTOF only:

Special Program for De-Barcoding (see tutorial) (modified Dec 2, 2011 - you can now leave in the barcoded data (check) or leave it out.

64 bit version PlateAnalyzer: (STABLE)

Here is the 64 bit version. This is an automatic download

1. 64 bit version: Download

64 bit version PlateAnalyzer: (Development version may crash or behave like a 2 year old....)

Here is the 64 bit version version under development. Select the most recent version. You may bave to manually move this to a different location as PA assumes a fixed location ( Program Files (x86)/PUCL ). This is an automatic download