Last Updated: 9 Feb 2014 (Check what has been updated)

This software is provided to GSK on a demonstration use only. Please do not distribute any files. The software and data are provided under confidentiality between PUCL and you. Please respect this. Email JPR if you have problems (jpr@flowcyt.cyto.purdue.edu)

1. Video Tutorial onAccuri Autowell finder

Please download the following files.

I suggest you play the tutorials while playing with the files. This way you can follow along with the tutorial. There are more basic tutorials at our EDUCATION page, Tutorials. They may be helpful.

PlateAnalyzer Programs - there are three files - the main file is platAn.exe, there is an INI file which records your file history, and your license file. All three files must be in the same directory. These are coded for your use only. You may use as many copies as you want for your lab use, on as many computers as you want, but they will all be coded with your name. If you want a collaborator to have a copy please send JPR an email.

Download the parameters and plate layout files and I suggest you create a directory to identify them as such.

Some nice introduction features in tutorials are HERE

Full Plate Examples for Drug Dose Responses

These examples are useful as you can download these files (particularly plate 58) and many of the videos use this file. Make sure you download the concentration file and the PAP files as well. Then follow the videos by copying what they do....you should see the exact same thing.

• Example plate 58 - 384 well plate with JC-1 (ZIP file unzip to use)
• Example plate 65 - 384 well plate with Redox (Zip file unzip to use)

Parameter & Plate Layout

Example drug concentration CSV file (use for plates 58 & 65)

Example PAP file for Plate 58 (simple layout)

Example PAP file for Plate 58 (advanced layout)

Drug Concentration File (CSV) for 58 and 65

Example PAP file for Plate 65 (advanced layout)

Example PAP file for Plate 65 (Very advanced layout) this layout shows how you can create an entire report in a single protocol to produce all 96 graphs.This is pretty cool!

Special issue for ACCURI file converter in PlateAnalyzer

Accuri files stop at 1 million cells and keep creating files with 1 million cells. If you want to look at these with PlateAnalyzer you have to combine them and set the number of wells etc. There is a special setting in PlateAnalyzer to do this.

Here is a Tutorial for this feature

Special Issues for CyTOF data

To be updated

Parameter & Plate Layout for Cytof

Vertical Drug Concs. CSV file

Vertical Drug Concs (reversed!!) checking if this is correct!!

Example complex layout for Sunitinib data set (works for others)

More compelex file as on 11/29 call - Sunitinib data set (ditto)

Sunitinib data file DE-BARCODED (418meg zip)

New Logic Map Protocol for March 8, 2012 with New DRUGBOXES

Tutorials (mainly Cytof)

1. Initial Ideas (Sept 26)

2. Here are the general  tutorials

3. Specific tutorial for Bodenmiller data only (37 meg)

4. Decoding Tutorial is here (4.6 meg)

5. My Video of Checker (2 dec) (5meg)

6. My Video of Starsnstripes (2 dec - low audio sorry!) (7meg)

7. March 8, 2012 - New Drugbox controls

Programs

32 bit:

• PlatAn.exe   (the latest version of the main program)
• PlatAn.ini   (can be deleted any time but it remembers what you have done - if you have major problems and the program seems possessed, just deleted this file and start again). The main program installation is in c:/program files (x86)/PUCL/Plate Analyzer (x86 for 64 bit) and c/:program files/PUCL/Plate Analyzer (for 32bit computers). The download is to your /users/name directory (windows 7 does not allow editing files in main program directory) but the program is run from the c:/program files  directory and this is where you MUST place your LIC file.

64 bit version PlateAnalyzer:

Here is the 64 bit version. This is an automatic download

1. 64 bit version: Download

64 bit version PlateAnalyzer: (Development version may crash or behave like a 2 year old....)

Here is the 64 bit version version under development.  Select the most recent version. You may bave to manually move this to a different location as PA assumes a fixed location ( Program Files (x86)/PUCL  ).  This is an automatic download - Pick the latest date. These versions have lots of new features but sometimes are unstable.

1. 64 bit version: Download

• Platan.lic  (Jepras only) (your license file which must remain with the exe) MUST BE IN THE SAME DIRECTORY AS THE PLATAN.EXE file. You may download as many copies as you need but they will all have the same name on it.