The following are CyTutorials(TM) I am developing to bring a new level of education to the flow community. I have seen a lot of rubbish out there, and over 25 years of providing materials for the field, I decided it is time to do something much better than is being fed to our new cytometry community members. I would like your feeback tell me errors or omissions...I want these to be absolutely correct - including typos!!!!
These are not for public distribution. The following are private links on the CYTOMETRYMAN Youtube channel. Use only with permission. Comments and suggestions appreciated. Send them directly to me wombat@purdue.edu
Paul Robinson
1. Introduction to Flow Cytometry (for general public) (5 Minutes)
2. Cell Sorting
4. Size Matters
5. Excitation & Emission
6. Principles of Fluorescence
7. Fluidics of a Flow Cytometer
8. Light Souces in Flow Cytometry
9. What is a histogram
10. Listmode and FCS data structure
11. Prinicples of gating
12. Cell fixation and application in flow
13 Sample background and its impact on quality data
14. Doublet discrimination
15. Why are negative populations useful?
16. How does a PMT work?
17. Log vs Linear signals and data transformations
18. Titration of antibodies
19. Intracellular staining
