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PUCL Job#1377 - Core Manager - LA (posted July 18, 2022)

Flow Cytometry Core Manager for Broad CIRM Center

Apply Keck School of Medicine Los Angeles, California

 

https://usccareers.usc.edu/job/los-angeles/flow-cytometry-core-manager-for-broad-cirm-center/1209/31548229136

The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC and the Department of Stem Cell Biology and Regenerative Medicine is in search of a Laboratory Manager to manage the Flow Cytometry Facility.

The Flow Cytometry Facility provides the research community with state-of-the-art technology to selectively phenotype and isolate specific populations of cells. Using fluorescent probes and transgenic proteins, researchers monitor several aspects of their cell populations, including phenotype, cell viability, proliferation, cell cycle and cell activation. Additionally, cell purification by electrostatic cell sorting enables cutting-edge research on low frequency cells. This essential resource aides investigators performing research in cancer, cancer-related areas, stem cell biology and regenerative medicine. With the increasing number of assays being developed that utilize flow cytometry, this resource supports a diverse group of investigators at USC and in the Los Angeles region.

The facility has been used to address questions in adult and embryonic stem cell research, regenerative medicine, cellular immunology, cell biology, chemical carcinogenesis, radiology, human tumor cell cycle kinetics, mechanisms of drug action, and differences between patient samples and normal donors.

Essential duties and responsibilities include:

  • Oversee daily operation and instrument maintenance of Flow Cytometry Facility
  • Hiring, training, assigning duties, scheduling, and supervision of facility staff.
  • Operating flow cytometry instrumentation for data collection and cell sorting
  • Facility billing (monthly), budget (annual), and usage reporting (monthly & annual)
  • Attending meetings and generating data for monthly and yearly reports for Norris Cancer Center
  • Purchasing of supplies, equipment, and service contracts. Negotiating pricing as applicable.
  • Develop, optimize, and consult on investigators experimental assays.
  • Flow cytometry lecture and lab for Dept Masters Class
  • Flow cytometry lecture and lab for PCC Broad program and also high school summer students
  • Develop, assess, and implement policies and procedures for the Flow Core
  • Writing grants to acquire new and complementary technology
  • Answer users scheduling and billing questions
  • Maintenance of aseptic conditions for cell sorting
  • Data management, .fcs file transfer, FlowJo software oversight
  • Contribute to investigators grant applications and publications through data cquisition/analysis, writing, or review as appropriate.
  • Attend meetings/conferences/functions as appropriate to represent the Flow Core and answer questions

Job Qualifications and Preferred Experience:

  • Experience with multicolor panel design.
  • Experience with DIVA software.
  • Previous experience working in a Shared Resource Flow Core, specifically with instrument troubleshooting/maintenance would be highly desirable.
  • Ability to serve as the technical and scientific knowledge “expert” regarding flow cytometry.
  • Experience with flow cytometry instruments from various companies would be desirable.
  • Communication and social skills: we are here to serve our facility users and so we need to ask questions and communicate clearly, but be generally friendly and helpful.
  • Comfortable giving presentations/speaking in public: either through lectures or speaking in department meetings.
  • Ability to take on duties/roles not already defined but clearly appropriate to the Flow Core and the position: website updates/improvements/content, coordinating seminars/speakers for flow cytometry related content, etc.

Performs other related duties as assigned or requested. The university reserves the right to add or change duties at any time.


Oversees the operation and maintenance of one or more laboratory facilities for a school or academic or research department. Provides technical laboratory expertise to faculty, research staff and graduate or undergraduate students in the design and execution of experiments.



REQ20119330 Posted Date: 06/23/2022

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