Senior Associate Scientist, Flow Cytometry - (Internal REQ #1617002)
The Inflammation & Immunology Research Unit is seeking a highly motivated scientist to work in our expanding flow cytometry lab. The applicant should have a solid background in flow cytometry and cell sorting. The qualified candidate will be responsible for independently running FACS-based and other assays to support many early and late-stage projects. In addition, the candidate will help train others on flow cytometry concepts including planning experiments, proper use of equipment, and correct data analysis. The candidate should have a desire to build on their knowledge of flow cytometry and be excited to take on new technologies and methods pertaining to developing a state-of-the-art flow cytometry lab.
MAIN RESPONSIBLITIES
· QC instruments on a daily basis and troubleshoot problems; build machine configurations and change configurations as needed.
· Help train other users on all aspects of flow cytometry from planning experiments, acquisition and analysis.
· Perform cell sorting and manage sort requests.
· Characterize and qualify reagents to be used in assays, including FACS antibody panels and titers.
· Develop and perform multicolor staining assays for primary human and mouse phenotyping experiments.
· Work independently, seeking help as needed, to conduct various FACS-based screening assays for small molecule and protein therapeutics.
· Perform data analysis using FACSDiva and FlowJo applications.
· Interpret and summarize experimental data and report summaries in team meetings, as needed.
· Familiarity with emerging technologies such as CyTOF and desire to expand knowledge past traditional flow cytometry.
· Experience working with liquid handling devices, or cytometers as part of a robotic automation suite, desirable.
BASIC QUALIFICATIONS
· BA or BS degree with 2+ years flow cytometry experience in appropriate scientific area related to position. A degree in the following is strongly preferred: Cell biology, molecular biology, microbiology, immunology or biochemistry.
PREFERRED QUALIFICATIONS
· Have an understanding of flow cytometry fundamentals and experience with analyzers including: BD LSRFortessa, Miltenyi MACSQuant and Intellicyt iQue Screener PLUS.
· Experience cell sorting on a BD FACSAria and Sony SH-800 machines.
· Comfortable developing and running 6+ color FACS panels.
· General ability working with Windows and MAC operating systems and computer hardware/devices.
· Software proficiency: Microsoft Office, FACSDiva, FlowJo, MACSQuantify, Forecyte and Graphpad Prism.
EEO Statement
EEO & Employment Eligibility
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Additional Offer Details:
· Last Date to Apply: December 5, 2017
· This job is a Pfizer Exempt US Grade: 004
· Additional Location Information: Cambridge, MA
· Eligible for Employee Referral Bonus
Interested candidates please apply at:
