Senior Scientist – Flow Cytometry

Medical Science & Computing (MSC) is an exciting growth oriented company, dedicated to providing mission critical scientific and technical services to the Federal Government.  We have a distinguished history of supporting the National Institutes of Health (NIH) and other government agencies. MSC offers a dynamic and upbeat work environment, excellent benefits and career growth opportunities.

We attract the best people in the business with our competitive benefits package that includes medical, dental and vision coverage, 401k plan with employer contribution, paid holidays, vacation, Medical and Flexible Spending Accounts, Pre-Tax Transit Assistance and tuition reimbursement. If you enjoy being a part of a high performing, professional service and technology focused organization, please apply today!

MSC is currently searching for a Senior Scientist – Flow Cytometry to support the National Institutes of Health (NIH). This opportunity is full-time, and it is on site in Bethesda, MD.

The Sr. Scientist will support the Flow Cytometry Section that offers a variety of techniques/applications which include:

• High speed flow cytometric sorting of up to four cell populations identified with up to 17 colors

• Flow cytometric analysis with up to five lasers and 28 colors

• Single cell cloning into 96-well plates, including sorting into PCR plates

• Intracellular calcium measurements using Indo-1, combined with surface markers

• Cell cycle analysis (using PI, DAPI, or Hoechst)

• FRET assays using GFP variant reporters or β−lactamase FRET-based reporter systems

• SP (Side Population) analysis for stem cell detection using Hoechst dye

• Flow Imaging applications, such as nuclear translocation, colocalization, phagocytosis, conjugate assays, etc.(see below), using the AMNIS Imagestream

• High speed flow cytometric sorting (up to nine colors) and analysis (up to 12

colors) on infectious (BSL3 agents) samples, either Select or non-Select Agents.

Duties and Responsibilities:

·      Conduct extensive interaction with numerous intramural investigators to provide advice on fluorochrome usage, suggestions for experimental protocols, advice on products for flow cytometry such as reagents and analysis software, and experimental design for flow cytometric approaches to a particular experiment or project, e.g. panel development.

·      Provide oversight to the Custom Antibodies Facility.

·      Supervise staff and troubleshoot instruments

• Establish and/or manage policies and procedures, including laboratory biosafety policies
• Consult with  investigators, including meetings prior to the initiation of new sorting experiments
• Education, both a formal introductory flow cytometry course and one-on-one training
• Manage consultation with instrument manufacturers
• Technology development and collaboration with investigators to facilitate novel applications
• Participation in NIH Select Agent and Biosurety Programs
• Provide  recommendations for instrument upgrade/replacement consistent with the overall goal of maintaining a state-of-the-art facility

·      Assist with overseeing  the operation of the Custom Antibodies Facility whose mission is to provide monoclonal antibody purification, hybridoma propogation and custom conjugation to fluorescent and non-fluorescent biomarkers.

Position Requirements:

·      PhD degree with 15+ years of relevant experience

·      Expert knowledge of flow cytometry technology and applications

·      Demonstrated supervisory experience.  Experience managing a Laboratory or a Section required.

·      Technical proficiency and theoretical knowledge of a wide variety of instrumentation

Please send cover letter and resume to elisa@mscweb.com

Medical Science & Computing is an Equal Opportunity/Affirmative Action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, or protected Veteran status.