
The Jackson Laboratory for Genomic Medicine, based in Farmington, CT is seeking a Flow Cytometrist responsible for independent oversight of the daily operations in the flow cytometry facilities to support JAX-GM Research.
The incumbent will be responsible for keeping current on relevant technology, analysis software and communicate this to the Faculty Partner and user base. In addition this position will present data in PI laboratory meetings, as well as present at appropriate interest groups within the Lab community. Primary duties will include: user training, consultation, protocol design, performing multi-color phenotyping experiments, cell sorting, data analysis, maintenance, trouble-shooting, and in some cases repair of the instruments in the flow facility
Key Responsibilities
· Provide the calibration, maintenance, troubleshooting, and any requested researcher assistance on all flow cytometry equipment (FACSAria Fusion Sorter, LSRFortessa X-20, FACSCanto, and Sony SP6800 Spectral Analyzer).
· Maintain current protocols and SOPs for all aspects of equipment operation and data analysis.
· Keep all equipment in good working condition and ready for use at all times including a well-stocked inventory of cytometry reagents to enable users to perform studies 24/7. Provide some troubleshooting or work with manufacturer’s Technical Support and Technical Engineers.
· Understand all operational aspects of the FACSAria cell sorter. Provide independent or operator-assisted sorting on a flexible schedule, as a primary job responsibility. This includes changing nozzle tips, finding the proper drop drive frequencies to optimize sort efficiency, perform calibrations, and evaluate daily standardization results to take appropriate actions in case of problems.
· Provide independent or operator-assisted acquisition and analysis of multi-color FACS samples in facilities BD and Sony machines (LSR-II, Canto, Fortessa, SP6800) and will be responsible for setting up and running and analysis of high level complexity FACS samples.
· Provide education and train new users to flow cytometry theory for instrument use, general facility etiquette, safety, some basic trouble-shooting, software function, and data interpretation & representation.
· Maintain technical currency, identify and assess developing technologies, platforms, reagents, and methods. Import and deploy as appropriate.
· Take an active role in understanding user interests by attending JAX-GM PI Lab meetings and seminars. Search out new techniques or innovations or antibody combinations the will provide maximum benefit to the researchers.
· Provide consultation abilities to take an experiment from conceptual goal towards instrument choice, design, color combinations, antibody choices, tissue collection procedure, staining, instrument operation, data analysis, interpretation, with a formal presentation to the user.
· Establish and meet Service Level Agreements including deliverables definitions, time lines and quality.
· Provide required reporting, attend Service Staff meetings and execute monthly billing.
· Process costing, deliver quotes, and develop fees structure for new deliverables.
· Contribute to budget development and management.
· Meet regularly with the Faculty Partner to discuss opportunities for research and development.
· Enhance Service capabilities through R&D activities funded through the service operating account, Res. Admin Account, or Director’s Innovation funds.
· Capability to investigate and assess new instrumentation. Also provide early access to researchers through demos or other facilities.
Required Skills:
· MS degree or an equivalently experienced B.S. degree in the Biological sciences or Biochemistry with proficient knowledge of genetics, physics, chemistry, instrumentation, immunology, and computers.
· Two to three years’ experience in providing Flow Cytometry with hands on experience with BD Sorters (FACSAria) and analyzers (LSR-II or Fortessa X-20) is required. Familiarity with additional FACS machines including Sony SP6800 is a plus.
· Demonstrated successful experience in formulating, implementing and evaluating complex plans and programs
· Demonstrated ability to successfully manage multiple provides to completion.
· Demonstrated ability to be a positive team player across diverse internal and external organizations
· Experience using specialized analysis software (FlowJo, CellQuest, DiVa, Ideas (ISX) as well as Microsoft Office products. Proficiency on Macintosh and PC computers.
· Excellent personal communication skills for both individual and group settings. Demonstrate patience, a willingness to help others, an ability to explain complex principles clearly and concisely to all experience levels.
· Training in bacteriological, viral, chemical and physical safety. Able to work safely in a BSL2 environment.
The Jackson Laboratory is recognized as the leading mammalian genetics research institute focused on mouse genetics and disease research, and a unique resource for the scientific community. As part of our research mission, we develop and offer advanced resources and services to the scientific and biomedical research community. The Jackson Laboratory offers an environment where professional growth and development are assigned the highest priority. We offer a generous wage and benefits package to encourage members of our team to advance to levels of expertise while concurrently supporting clearly defined mission, vision and values.
For more information or to apply online, please visit our career board at www.jax.org/careers