(no subject)

From: Maher3148@AOL.COM
Date: Tue Jan 11 2000 - 17:10:32 EST 

I am planning to study intracellular cytokine expression in lymphocytes 
following activation with PMA + ionomycin, but have concerns about reports 
of CD4 down regulation following activation.  I would like to know if this 
is an 'all or none' phenomenon or if the CD4 remains expressed at a level 
sufficient to permit delineation from true CD4 negative cells. If you have 
experience here, I would appreciate your comments. Thanks. 

  please reply to: kmaher@med.miami.edu

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