Dear Flowers So many precious answers from this group - thus this question: We have for some time looked into apoptosis using among other parameters Annexin - V. Generally this works well using A-V-FITC from Pharmingen. We now want to bind onto A-V (non-FITC-tagged) bound to PS, the mouse monoclonal antibody Mab 3184 Chemicon clone RUU-WAC2A (designed for ELISA or immunohistochemistry). So far we have had no success as evidenced from binding a PE tagged antimouse antibody (rabbit) to the PS-A-V-Mab3184 complex. We have searched for other A-V antibodies, but all suppliers appear to use the same clone, RUU-WAC2A. We sure appreciate all comments / suggestions. Thanks. Peter Kierulf e-mail: peter.kierulf@ioks.uio.no
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