Cell surface staining and ethanol fixation

From: oes@rsp.is
Date: Thu Apr 13 2000 - 05:42:02 EST

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hi there

I would like to know wether it is is ok to stain cells for cell surface
markers fix them with ethanol keep them in a freezer for one month and
then stain for DNA content using PI.  What effect would that have on my
cell surface markers in the Flowcytometry to fix the cells in ethanol

Hope some one will respond a.s.a.p.

yours

Ólafur E Sigurjónsson
oes@rsp.is

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