I have had only limited work in this but usually EtOH fixation after a congugated primary stain does not work very well. What I used to do is stain cells, fix in 1% paraformaldehyde, wash once and then fix in EtOH, that seemed to preserve the AB stain much better while still giving good CVs for the DNA. I have never tried storing the cells after EtOH fixation. Brian Newsom Technical Application Specialist BD/Phamingen -----Original Message----- From: oes@rsp.is To: cyto-inbox Sent: 4/13/00 5:42 AM Subject: Cell surface staining and ethanol fixation hi there I would like to know wether it is is ok to stain cells for cell surface markers fix them with ethanol keep them in a freezer for one month and then stain for DNA content using PI. What effect would that have on my cell surface markers in the Flowcytometry to fix the cells in ethanol Hope some one will respond a.s.a.p. yours Ólafur E Sigurjónsson oes@rsp.is
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