Dane Wittrup writes: >Does anybody have their flow instrument set up for excitation from 280-300 >nm? We'd like to excite a lanthanide bound in calmodulin, using Trp as the >donor to the ion. There aren't a lot of people who have lasers that will do those deep UV lines - the only publication in this area which comes to mind was from the Jovins in Goettingen in the late 1970's, looking at Trp fluorescence in proteins. However, if you're really interested and have the laser (which would presumably be on an optical bench) and optical filters, I could bring over the rest of a cytometer. You might also be able to scrounge the do-it-yourself cytometer parts from Penny Chisholm's lab, which is even closer to you. Of course, the real problem might be the lanthanide lifetime; as I recall, this runs into many microseconds, while the typical dwell time in the beam of a cytometer is only a few microseconds. Call if you're interested (617-783-8392 or 617-965-6044). -Howard
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:16 EST