>I am planning to study intracellular cytokine expression in lymphocytes >following activation with PMA + ionomycin, but have concerns about reports >of CD4 down regulation following activation. I would like to know if this >is an 'all or none' phenomenon or if the CD4 remains expressed at a level >sufficient to permit delineation from true CD4 negative cells. If you have >experience here, I would appreciate your comments. Thanks. We certainly see strong downregulation of CD4 after PMA/Iono - however, if you are able to use PE-, APC-, or especially CyChrome-conjugated Ab, you should be able to get enough separation to reliably gate on the CD4 population. AW Andrew D. Wells, Ph.D. University of Pennsylvania Department of Medicine 728 Clinical Research Building 415 Curie Boulevard Philadelphia, PA 19104 (215) 573-1840 (office) (215) 898-1951 (lab) (215) 573-2880 (FAX) adwells@mail.med.upenn.edu
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:03 EST