(no subject)

From: Diana Martin (zigra@hotmail.com)
Date: Mon Aug 14 2000 - 10:38:47 EST


Does anyone ultracentrifuge their labeled primary antibodies or MHC
tetramers before use in cell surface staining?  If so, can someone pass
along what conditions you use (ie how many g's and for how long)?  We have a
desktop airfuge.  I can't find a chart anywhere telling what size proteins
pellet at what speed.  If anyone has this info, I would greatly appreciate
it.

Thanks

Diana Martin
Post-doctoral Fellow
Center for Tropical and Emerging Global Diseases
University of Georgia
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