Send reply to: pmcclosk@mail.ncmc.edu
Organization: Nassau Health Care Corporation
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Subject: Antibody Binding Capacity Problems
In our laboratory we are currently using Quantum-PE and Quantum Simply
Cellular kits, from Flow Cytometry Standards Corporation, to measure
Antibody Binding Capacity (ABC)of Fc receptors on human neutrophils. We
are getting very high ABC values which are 3 to 4 times higher than the
values reported in the literature by standard methodologies. According
to the technical representative at FCSC/Bangs Laboratories, we are doing
the quantitation correctly but the cells are just extremely bright.
My question is:
Does anyone have experience using these products? In particular,
antibodies labeled with PE and not FITC?
Thank you. I would appreciate any suggestions that you may have.
Pam McCloskey
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Pamela McCloskey
Research Scientist II
Nassau Health Care Corporation
Department of Pediatrics
2201 Hempstead Turnpike
East Meadow, NY, 11554
Phone: (516) 572-6205
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