Antibody Binding Capacity Problems

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We are currently using Quantum-PE and Quantum Simply Cellular kits, from
Flow Cytometry Standards Corporation, to measure Antibody Binding
Capacity (ABC)of Fc receptors on human neutrophils.  We are getting very
high ABC values which are 3 to 4 times higher than the values reported
in the literature by standard methodologies.  According to the technical
representative at FCSC/Bangs Laboratories, we are doing
the quantitation correctly but the cells are just extremely bright.

My question is:
    Does anyone have experience using these products?  In particular,
antibodies labeled with PE and not FITC?

Thank you.  I would appreciate any suggestions that you may have.

Pam McCloskey

********************************
Pamela McCloskey
Research Scientist II
Nassau Health Care Corporation
Department of Pediatrics
2201 Hempstead Turnpike
East Meadow, NY, 11554
Phone: (516) 572-6205

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