In my experience you can count on the CD4 disappearing. Depending on your
assay you might use CD8-3+ cells to delineate CD4+ cells of interest
F Preffer
At 05:10 PM 01/11/2000 EST, Maher3148@AOL.COM wrote:
>
>I am planning to study intracellular cytokine expression in lymphocytes
>following activation with PMA + ionomycin, but have concerns about reports
>of CD4 down regulation following activation. I would like to know if this
>is an 'all or none' phenomenon or if the CD4 remains expressed at a level
>sufficient to permit delineation from true CD4 negative cells. If you have
>experience here, I would appreciate your comments. Thanks.
>
> please reply to: kmaher@med.miami.edu
>
>
>
>
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Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Room 7140
149 13th Street
Massachusetts General Hospital-East
Charlestown, MA 02129
v(617) 726-7481 fax (617) 724-3164
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