Hi every body, I would like to know if it is ok to use a 48 hr old peripheral blood that was collected in EDTA for lymphocyte subsets using the MultiTEST reagents (using the CD45 vs SSC gating strategy). Currently we are rejecting such specimens on the bases that EDTA is not a great anti-coagulant to test for lymphocyte subsets in blood that is older than 24 hr. any comments or recommendations are welcome. Faisal Rawas Senior-in -charge Flow Cytometry Lab King Faisal Hospital Riyadh, KSA ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
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