Dear all, I am new to the list, but am interested in your input. > > I have some questions about the relationship of FSC to size. I have been > trying to evaluate a B cell line for their mitochodrial potential using > DiOC6, a potential sensitive dye. When I induce cell death in this line, I > see two populations that differ in about 40 channels (mean) on FL-1 (log > scale, FL1-H) for DiOC6 staining.. I also see a shift in the population by > FSC by about 2 fold (600 to 300) on a linear scale. I want to be sure that > the change in DiOC6 fluorescence is related to the change in potential and > not size. > > Is there a relatively straight forward relationship between size and FSC? > Does 2 fold mean 2 fold diameter? volume? neither? > > I never see a cell with high FSC that has low potential and the opposite > is true also.. > > Thanks so much. > Ellen Ellen Cahir McFarland Postdoctoral Fellow Kieff Lab Brigham and Women's Hospital and Harvard University 181 Longwood Ave 8th floor MCP Boston, MA 02115 phone 617-525-4270 fax 617-525-4251 ecahir@rics.bwh.harvard.edu
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