Key Papers in Cytometry
- 1941
- Nucleic acids shown necessary for protein synthesis
- Caspersson, T. Studien über den Eiweiβumsatz der Zelle. Naturwissenschaften 1941; 29:3 33-43.
- 1941
- Uterine cancer detection
- Papanicolaou, GN., Traut, HF. The Diagnostic Value of Vaginal Smears in Carcinoma of the Uterus. Am J. Obst Gynec 1941; 42:193.
- Papanicolaou, GN. Some Improved Methods for Staining Vaginal Smears. J Lab Clin Med 1941; 26:1200.
- 1947
- Photoelectric particle counting
- Gucker, FT, Pickard, HB, and O’Konski, CT. A Photoelectric Instrument for Comparing the Concentrations of Very Dilute Aerosols, and Measuring Low Light Intensities. J. Am. Chem. Soc. 1947: 69, 2, 429-438.
- Gucker, FT, O’Konski, CT, Pickard, HB, and Pitts, JN. A Photoelectronic Counter for Colloidal Particles. J. Am. Chem. Soc 69, 10, 2422-2431, 1947.
- 1949
- Particle counting by Coulter volume
- Coulter, WH. Means For Counting Particles Suspended In A Fluid. U.S. Patent #2,656,508. Application August 27, 1949. Patented October 20, 1953.
- 1950
- DNA and RNA shown to increase in actively growing cells
- Caspersson, T. Cell Growth and Cell Function, A Cytochemical Study. 1950. Norton, NY.
- 1950
- Uterine cancer detection using fluorescence microscopy
- Friedman, HP Jr. The Use of Ultraviolet Light and Fluorescent Dyes in the Detection of Uterine Cancer by Vaginal Smear. Am J Obst Gynec 1950; 59:852.
- 1953
- Hydrodynamic focusing for reproducible delivery of cells in a fluid
- Crosland-Taylor, PJ. A Device For Counting Small Particles Suspended In A Fluid Through A Tube. Nature 1953:171, 37-38.
- 1955
- Automated scanning instrument for screening cytological smears
- Tolles WE. The Cytoanalyzer: An Example Of Physics In Medical Research. Trans N Y Acad Sci 1955; 17:250–256.
- 1963
- Fluorescence for quantitation
- Rotman, B, Zderic, JA, and M Edelstein. Fluorogenic Substrates for Β-D-Galactosideases and Phosphatases Derived from Fluorescein (S,6-Dihydroxyfluoran) and its Monomethyl Ether. Proc Natl Acad Sci 1963; 50(1): 1-6.
- 1964
- Acridine Orange differentiation of leukocytes
- Hallerman, L, Thom, R, Gerhartz, H. Elektronische Differentialzählung von Granulocyten und Lymphocyten nach intravitaler Fluorochromierung mit Acridinorange. Verh Deutsch Ges Inn Med 1964; 70:217.
- Kosenow, W. Die Fluorochromierung mit Acridinorange, eine Methode zur Lebendbeobachtung gefärbter Blutzellen. Acta Haemat 1952; 7:217.
- 1964
- Need for Automated Imaging Established
- Ingram, M, and Preston Jr., K. The Importance of Automatic Pattern Recognition Techniques in Early Detection of Altered Blood Cell Production. Ann NY Acad Sci 1964; 113:1066.
- 1965
- Cell sorting
- Fulwyler, MJ. Particle Separator. U.S. Patent #3,380,584. Application June 4, 1965. Patented April 20, 1968.
- Particle separator in principle capable of separating by volume, optical density or fluorescence
Fulwyler, MJ. An Electronic Particle Separator with Potential Biological Application. Los Alamos Scientific Laboratory Annual Report of the Biological and Medical Research Group (H–4) of the Health Division, July 1964 through June 1965, Written July 1965.
- Fulwyler, MF. Electronic Separation of Biological Cells by Volume. Science 1965; 150: 910-911.
- 1965
- Spectrophotometry of cells
- Kamentsky, LA, Melamed, MR and Derman, H. Spectrophotometer: New Instrument for Ultrarapid Cell Analysis. Science 1965; 150:630-631.
- 1966
- Fluorogenic esters in mammalian cells
- Rotman, B and Papermaster, BW. Membrane Properties of Living Mammalian Cells as Studied by Enzymatic Hydrolysis of Fluorogenic Esters. Proceedings of the National Academy of Sciences of the United States of America, 1966, Vol. 55, No. 1, 134-141.
- 1967
- Fluorescence Flow cytometry – First paper
- Van Dilla, MA, Mullaney, PF, and Coulter, JR. The Fluorescent Cell Photometer: A New Method for the Rapid Measurement of Biological Cells Stained with Fluorescent Dyes. Los Alamos Scientific Laboratory Annual Report of the Biological and Medical Research Group (H–4) of the Health Division, July 1966 through June 1967, Written September 1967.
- 1968
- Automated Imaging
- Ingram, M, Norgren, PE, Preston Jr., K. Automatic Differentiation of White Blood Cells. Image Processing in Biological Science, D.M. Ramsey (ed.), University of California Press, Los Angeles, pp. 97-117, 1968.
- Wald N, and Preston Jr., K. Automatic screening of metaphase spreads for chromosome analysis. Image Processing in Biological Science, D.M. Ramsey (ed.), University of California Press, Los Angeles, pp 9-34, 1968.
- Ingram, M, and Preston Jr., K. Automatic Analysis of Blood Cells. Sci Amer 1970; 223(5):72.
- 1968
- Fluorescence flow cytometry patent
- Göhde W. Automatisches Meβ – und Zählgerät für die Teilchen einer Dispersion. German Patent #DE1815352, priority date Dec. 18, 1968.
- 1968
- Scanning vs Flow for Cancer Cytology
- Wied, GL and Bahr, GF, Eds. Automated Cell Identification and Cell Sorting. 1970. Academic Press, NY. (Proceedings of 1968 Conference in Chicago).
- 1969
- Fluorescence Flow Cytometry – Third paper
- Hulett, HR, Bonner, WA, Barrett, J, and Herzenberg, LA. Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intracellular Fluorescence. Science 1969; 166: 747-749. Submitted 18 August, 1969.
- 1969
- Fluorescence Flow cytometry – Fourth paper
- Van Dilla, MA, Trujillo, TT, Mullaney, PF, and Coulter, JR. Cell Microfluorometry: A Method for Rapid Fluorescence Measurement. Science 1969; 163:1213-1214. Submitted 24 October, 1969.
- 1969
- First paper describing light scatter
- Mullaney, PF, Van Dilla, MA, Coulter, JR and Dean, PN. Cell Sizing: A Light Scattering Photometer for Rapid Volume Determination. Review of Scientific Instruments. 1969; 40(3): 1029-1032.
- 1972
- Fluorescence Activated Cell Sorting
- Bonner, WA, Hulett, HR, Sweet, RG, and Herzenberg, LA. Fluorescence Activated Cell Sorting. Review of Scientific Instruments 1972; 43 (3): 404-409.
- 1973
- Doublet Discrimination Patent
- Göhde W. Process for automatic counting and measurement of particles. US patent #4,021,117, priority date Jun 23, 1973 (DE).
- 1975
- Monoclonal Antibodies – Invention
- Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature Lon. 1975; 256:495-497.
- 1977
- Monoclonal Antibodies – First Use in Flow Cytometry
- Williams, AF, Galfrè, G, and Milstein C. Analysis of Cell Surfaces by Xenogeneic Myeloma-Hybrid Antibodies: Differentiation Antigens of Rat Lymphocytes. Cell 1977; 12:663-673.
- 1977
- Two-color Fluorescence Compensation
- Loken, MR, Parks, DR, Herzenberg, LA. Two-color immunofluorescence using a fluorescence-activated cell sorter. J. Histochem. Cytochem. 1977; 25: 899-907.
- 1979
- Flow imaging
- Kachel, V, Benker, G, Lichtnau, K, Valet, G and Glossner, E. Fast Imaging In Flow: A Means Of Combining Flow-Cytometry And Image Analysis. J Histochem Cytochem. 1979; 27(1):335-41.
- 1984
- Convention on Nomenclature for DNA Cytometry – Guidelines for analysis
- Hiddemann, W, Schumann, J, Andreeff, M, Barlogie, B, Herman, CJ, Leif, RC, Mayall, BH, Murphy, RF, and Sandberg, AA. Convention On Nomenclature For DNA Cytometry. Cytometry 5(5) 1984, 445-446.
- 1984
- Three-color immunofluorescence
- Parks DR, Hardy RR, Herzenberg LA. Three-Color Immunofluorescence Analysis Of Mouse B-Lymphocyte Subpopulations. Cytometry 1984, 5(2):159-68.
- 1987
- Dual-beam high-speed sorting
- Gray, JW, Dean, PN, Fuscoe, JC, Peters, DC, Trask, BJ, van den Engh, GJ, and Van Dilla, MA. High-Speed Chromosome Sorting. Science 1987; 238:323-329.
- 1990
- Data File Standard (FCS 2.0)
- Data File Standards Committee of the Society for Analytical Cytology. Data File Standard for Flow Cytometry. Cytometry 1990; 11:323-332.
- 1995
- 5-color flow cytometry
- Roederer, M, Bigos, M., Nozaki, T., Stovel, RT, Parks, DR and Herzenberg, LA. Heterogeneous
Subsets Revealed Calcium Flux in Peripheral T Cell by Five-Color Flow Cytometry Using Log-Ratio Circuitry. Cytometry 1995; 21:187-196.
- 1997
- 8-Color, 10-parameter flow Cytometry
- Roederer, M, De Rosa, S Gerstein, R, Anderson, M, Bigos, M, Stovel, R, Nozaki, T, Parks, DR, Herzenberg, L, and Herzenberg, L. 8 Color, 10-Parameter Flow Cytometry To Elucidate Complex Leukocyte Heterogeneity. Cytometry 1997; 29:328–339.
- 2001
- 11-color, 13-parameter flow Cytometry
- De Rosa, S.C., Herzenberg, L.A. & Roederer, M. 11-Color, 13-Parameter Flow Cytometry: Identification Of Human Naive T Cells By Phenotype, Function, And T-Cell Receptor Diversity. Nat. Med. 2001; 7: 245−248.
- 2004
- Seventeen-colour flow cytometry
- Perfetto SP, Chattopadhyay PK, Roederer M. Seventeen-Colour Flow Cytometry: Unravelling The Immune System. Nat Rev Immunol. 2004; 4(8):648-55.
