Has anyone used anything other than PI to do viability staining on the FLOW? 7-AAD? I have a researcher using PI and AO and they co-stain ALL the cells, virtually 100%, in what they believe to be live samples of Chondrocytes. Any hints? Does the collagenase used to break up the matrix of the cartilage cause permeabilization? I'm kind of at a loss to explain to him what is happening. Any better dye for this application? Thanks in advance! Susan DeMaggio, MS BSMT(ASCP)QCym Resource Manager Optical Biology Shared Resource University of California, Irvine Irvine, CA 92697-2275 phone 949-824-4110 fax 949-824-3571 <http://mamba.bio.uci.edu/~suedemag/OBCore/index.htm>http://mamba.bio.uci.e du/~suedemag/OBCore/index.htm
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:10 EST