Hi Sue, You can use HO33258 (if you have a UV laser), if you keep the cells on ice, and the histogram distribution will be similar to what you'd expect using PI. You can look at the cells (kept on ice) within a half hour and have excellent (1-2 log) differentiation between live and dead. I presented a poster on this at ISAC XIX directed towards users of arc lamp single detector instruments, but it will work fine in a multilaser set-up. Simply add HO33258 to an iced cell suspension at 1ug/ml final concentration. Analysis can be immediate after addition of dye. Peter > ---------- > From: Sue DeMaggio[SMTP:suedemag@uci.edu] > Sent: Monday, March 08, 1999 12:50 PM > To: Cytometry Mailing List > Subject: Viability / nuclear stain > > Has anyone used anything other than PI to do viability staining on the > FLOW? 7-AAD? > > > I have a researcher using PI and AO and they co-stain ALL the cells, > virtually 100%, in what they believe to be live samples of > Chondrocytes. Any hints? Does the collagenase used to break up the > matrix of the cartilage cause permeabilization? I'm kind of at a loss > to explain to him what is happening. Any better dye for this > application? > > Thanks in advance! > Susan DeMaggio, MS BSMT(ASCP)QCym > Resource Manager > Optical Biology Shared Resource > University of California, Irvine > Irvine, CA 92697-2275 > phone 949-824-4110 > fax 949-824-3571 > > http://mamba.bio.uci.edu/~suedemag/OBCore/index.htm >
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