At 11:50 AM 1999-03-08 -0800, Sue DeMaggio wrote: >Has anyone used anything other than PI to do viability staining on the FLOW? 7-AAD? >I have a researcher using PI and AO and they co-stain ALL the cells, virtually 100%, in >what they believe to be live samples of Chondrocytes. Any hints? Does the collagenase >used to break up the matrix of the cartilage cause permeabilization? I'm kind of at a >loss to explain to him what is happening. Any better dye for this application? >Any better dye for this application? It may not be PI you are seeing. AO has the unique property of shifting its emission to red when at a high enough concentration. This is often how it is used to visualize the transgolgi (I believe). It accumulates in the golgi and after a while turns red. I guess what I am suggesting is that you analyze your samples ASAP as the length of time the samples are exposed to the AO will determine how much red you will see. Good luck!
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