Hi.. Maybe try going a little "low tech" and have a look at them with trypan blue under a regular scope. I believe that should give similar results to PI if everything is going well... -Dave On Wed, 10 Mar 1999, Derek Schulze wrote: > >I have a researcher using PI and AO and they co-stain ALL the cells, > virtually 100%, in >what they believe to be live samples of Chondrocytes. > Any hints? Does the collagenase >used to break up the matrix of the > cartilage cause permeabilization? I'm kind of at a >loss to explain to him > what is happening. Any better dye for this application? > >Any better dye for this application? > > It may not be PI you are seeing. AO has the unique property of shifting > its emission to red when at a high enough concentration. This is often how > it is used to visualize the transgolgi (I believe). It accumulates in the > golgi and after a while turns red. I guess what I am suggesting is that > you analyze your samples ASAP as the length of time the samples are exposed > to the AO will determine how much red you will see. > > Good luck! >
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