PI depends on membrane integrity for exclusion. If PI doesn't work then any other fluorochrome dye that also is dependent on membrane integrity will probably do the same thing. You might try FDA, labels viable cells due to nonspecific esterase activity, but it might leak out of the live cells if they are permeable. Your best bet is to improve your preparation in order not to cause the live cells to become permeable. Deb Berglund On Mon, 8 Mar 1999, Sue DeMaggio wrote: > > Has anyone used anything other than PI to do viability staining on the FLOW? > 7-AAD? > > I have a researcher using PI and AO and they co-stain ALL the cells, virtually > 100%, in what they believe to be live samples of Chondrocytes. Any hints? > Does the collagenase used to break up the matrix of the cartilage cause > permeabilization? I'm kind of at a loss to explain to him what is happening. > Any better dye for this application? > > Thanks in advance! > Susan DeMaggio, MS BSMT(ASCP)QCym > Resource Manager > Optical Biology Shared Resource > University of California, Irvine > Irvine, CA 92697-2275 > phone 949-824-4110 > fax 949-824-3571 > > <http://mamba.bio.uci.edu/~suedemag/OBCore/index.htm>http://mamba.bio.uci.e > du/~suedemag/OBCore/index.htm
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