I recently had an interesting case. We received bone marrow on a 75 yo WF
with history of MDS. We noted immediately that she now has leukemia that is
+CD34, CD13, CD65 and CD10 but negative for CD33, CD71, CD11b, CD14, CD61,
CD41, CD42b, CD64, CD36, CD19, CD20, kappa, lambda, CD4, CD8, CD2, CD5,
CD16, CD56, and CD57. Unfortunately our MDS panel is set up to answer
protocol questions and doesn't have all leukemia antigens we would wish
(yes we have to worry about costs too). The blasts are weird, as expected
for MDS, and have scant cytoplasm, no granules and wild but small nuclei.
We then did some IP on the parrafin stuff and found the
following:myeloperoxidase -, Leu 22(CD43) + and Leu M1-CD15- weird spotty
+. TDT is pending. My question- Has any one seen CD65 on an ALL? What do
you think of this?
Maryalice
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:23 EST